Alexa Fluor 594 anti-ERK1/2 Antibody anti-ERK1/2

Clone: W15133B (See other available formats)
Regulatory Status: RUO
Other Names: Extracellular signal-regulated kinase 1/2, Mitogen-activated protein kinase MAPK3/MAPK1, p44ERK1/p42ERK2, p44-MAPK/p42MAPK, PRKM3/PRKM2, ERK
Isotype: Rat IgG1, κ
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Product Citations: publications

W15133B_A594_ERK1slash2_Antibody_ICC_041619 — HeLa cells were fixed with 4% paraformaldehyde (PFA) for 15 minutes, permeabilized with 0.5% Triton X-100 for three minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained with 2.5 µg/ml Alexa Fluor® 594 anti-ERK1/2 antibody (clone W15133B) overnight at 4°C. Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.

Cat #	Size	Price	Save
686903	25 µg	¥34,980

Description

ERK1/2 are members of the mitogen-activated kinases (MAPKs) that are serine/threonine protein kinases. ERK1/2 can be activated by a range of extracellular stimuli, such as mitogen, growth factors, neurotransmitters, chemokines, and cytokines, through receptor tyrosine kinases (RTK), G protein-coupled receptors (GPCRs), or protein kinase C (PKC). Upon stimulation, ERK1/2 are phosphorylated by the upstream kinase MEK on residues Thr202 and Tyr204 and in turn phosphorylate many other downstream molecules that are involved in a range of cellular processes such as cell proliferation, differentiation, motility and cell death.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human, Mouse, Drosophila
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Partial human ERK1 recombinant protein (180-379 a.a.) expressed in E. coli.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 0.5 - 2.5 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Application Notes: Reactivity to Drosophila was only verified with the purified format.

The drosophila homolog of mammalian ERK1/2 is the 43 kD mitogen-activated protein kinase ERK-A.
RRID: AB_2617023 (BioLegend Cat. No. 686903)

Antigen Details

Structure: ERK1 and ERK2 encode 42 and 44 kD protein kinases and share 85% homology.
Distribution: Nucleus and cytoplasm.
Function: ERK1/2 are broadly expressed protein kinases that are involved in proliferation, differentiation, motility, and cell death. In response to extracellular stimuli, ERK1/2 are phosphorylated at Thr202 and Tyr204 and in turn transduce signals to downstream substrates.
Interaction: Interacts with ADAM15, ARRB2, CANX, DAPK1, HSF4, IER3, MAP2K1/MEK1, MORG1, NISCH, SGK1, TPR, CDKN2AIP, PEA15, and MKNK2.
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Signal Transduction
Molecular Family: Protein Kinases/Phosphatase
Antigen References: 1. Futran AS, et al. 2013. Curr. Biol. 23:R972.
2. Mendoza MC, et al. 2011. Trends Biochem. Sci. 36:320.
3. Chambard JC, et al. 2007. Biochim. Biophys. Acta. 1773:1299.
4. Roux PP, et al. 2004. Microbiol. Mol. Biol. Rev. 68:320.
5. Torii S, et al. 2004. J. Biochem. 136:557.
6. Clark EA, et al. 1996. J. Biol. Chem. 271:14814.
Gene ID: 5595 View all products for this Gene ID 5594 View all products for this Gene ID
UniProt: View information about ERK1/2 on UniProt.org

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Other Formats

View All ERK1/2 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-ERK1/2	W15133B	WB,KO/KD-WB,ICC
Alexa Fluor® 594 anti-ERK1/2	W15133B	ICC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 06/24/2016

For Research Use Only. Not for diagnostic or therapeutic use.

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