- Clone
- C44Mab-5 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Hermes, Pgp-1, H-CAM, HUTCH-1, ECMR III, gp85, Ly-24
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![C44Mab-5_A594_CD44_Antibody_1_061220 C44Mab-5_A594_CD44_Antibody_1_061220](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/C44Mab-5_A594_CD44_Antibody_1_061220.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human paraffin-embedded colon cancer tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Sodium Citrate H.I.E.R. 1X at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of anti-human CD44 (clone C44Mab-5) Alexa Fluor® 594 (red) at 4°C overnight. Nuclei were counterstained with DAPI (green). The image was captured with a 10X objective. -
Human paraffin-embedded breast tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Sodium Citrate H.I.E.R. 1X at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of anti-human CD44 (clone C44Mab-5) Alexa Fluor® 594 (red) at 4°C overnight. Nuclei were counterstained with DAPI (green). The image was captured with a 10X objective.
Cat # | Size | Price | Save |
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397509 | 25 µg | ¥24,640 | |
397510 | 100 µg | ¥55,660 |
CD44 is a 80-95 kD glycoprotein also known as Hermes, Pgp1, H-CAM, or HUTCH. It is expressed on all leukocytes, endothelial cells, hepatocytes, and mesenchymal cells. As B and T cells become activated or progress to the memory stage, CD44 expression increases from low or mid levels to high levels. Thus, CD44 has been reported to be a valuable marker for memory cell subsets. High CD44 expression on Treg cells has been associated with potent suppressive function via high production of IL-10. CD44 is an adhesion molecule involved in leukocyte attachment to and rolling on endothelial cells, homing to peripheral lymphoid organs and to the sites of inflammation, and leukocyte aggregation.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
IHC-P - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Product Citations
-
- RRID
-
AB_2860987 (BioLegend Cat. No. 397509)
AB_2876720 (BioLegend Cat. No. 397510)
Antigen Details
- Structure
- Variable splicing of CD44 gene generates many CD44 isoforms, 80-95 kD
- Distribution
-
All leukocytes, epithelial cells, endothelial cells, hepatocytes, mesenchymal cells
- Function
- Leukocyte attachment and rolling on endothelial cells, stromal cells and ECM
- Interaction
- Endothelial cells, Epithelial cells, Leukocytes, Mesenchymal cells, Mesenchymal Stem Cells, B cells, Tregs
- Ligand/Receptor
- Hyaluronan, MIP-1β, fibronectin, collagen
- Cell Type
- B cells, Dendritic cells, Mesenchymal Stem Cells, Tregs
- Biology Area
- Cell Biology, Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
- Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
- Haynes BF, et al. 1991. Cancer Cells. 3:347.
- Goldstein LA, et al. 1989. Cell. 56:1063.
- Mikecz K, et al. 1995. Nat. Med. 1:558.
- Hegde V, et al. 2008. J. Leukocyte Biol. 84:134.
- Liu T, et al. 2009. Biol Direct. 4:40.
- Gene ID
- 12505 View all products for this Gene ID 960 View all products for this Gene ID
- UniProt
- View information about CD44 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All CD44 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-human CD44 | C44Mab-5 | FC,IHC-P |
PE anti-human CD44 | C44Mab-5 | FC |
APC anti-human CD44 | C44Mab-5 | FC |
Alexa Fluor® 647 anti-human CD44 | C44Mab-5 | FC,IHC-P |
Alexa Fluor® 594 anti-human CD44 | C44Mab-5 | IHC-P |
Alexa Fluor® 488 anti-human CD44 | C44Mab-5 | FC |
PE/Dazzle™ 594 anti-human CD44 | C44Mab-5 | FC |
PE/Cyanine7 anti-human CD44 Antibody | C44Mab-5 | FC |
FITC anti-human CD44 Antibody | C44Mab-5 | FC |
PerCP/Cyanine5.5 anti-human CD44 Antibody | C44Mab-5 | FC |
Alexa Fluor® 700 anti-human CD44 | C44Mab-5 | FC |
Spark Blue™ 550 anti-human CD44 (Flexi-Fluor™) | C44Mab-5 | FC |
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD44
Human peripheral blood lymphocytes were stained with purifie... Human paraffin-embedded breast tissue slices were prepared w... Human paraffin-embedded colon cancer tissue slices were prep... -
PE anti-human CD44
Human peripheral blood lymphocytes stained with CD44 (clone ... -
APC anti-human CD44
Human peripheral blood lymphocytes stained with CD44 (clone ... -
Alexa Fluor® 647 anti-human CD44
Human peripheral blood lymphocytes stained with CD44 (clone ... Human paraffin-embedded colon cancer tissue slices were prep... Human paraffin-embedded breast tissue slices were prepared w... -
Alexa Fluor® 594 anti-human CD44
Human paraffin-embedded colon cancer tissue slices were prep... Human paraffin-embedded breast tissue slices were prepared w... -
Alexa Fluor® 488 anti-human CD44
Human peripheral blood lymphocytes stained with CD44 (clone ... -
PE/Dazzle™ 594 anti-human CD44
Human peripheral blood lymphocytes stained with CD44 (clone ... -
PE/Cyanine7 anti-human CD44 Antibody
Human peripheral blood lymphocytes stained with CD44 (clone ... -
FITC anti-human CD44 Antibody
Human peripheral blood lymphocytes stained with CD44 (clone ... -
PerCP/Cyanine5.5 anti-human CD44 Antibody
Human peripheral blood lymphocytes stained with CD44 (clone ... -
Alexa Fluor® 700 anti-human CD44
Human peripheral blood lymphocytes stained with CD44 (clone ... -
Spark Blue™ 550 anti-human CD44 (Flexi-Fluor™)
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