Alexa Fluor® 594 anti-human CD44 isoform 9 (CD44v9) Antibody CD44, RV3

Clone: RV3 (See other available formats)
Regulatory Status: RUO
Other Names: CD44 variant 9, CD44 variant isoform 9
Isotype: Rat IgG2a, κ
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Product Citations: publications

RV3_A594_CD44v9_Antibody_100918 — Human paraffin-embedded colon cancer tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Sodium Citrate H.I.E.R. 1X at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of anti-human CD44v9 (clone RV3) Alexa Fluor® 594 (red) at 4°C overnight. Nuclei were counterstained with DAPI (green). The image was captured with a 10X objective.

Compare all formats See Alexa Fluor® 594 spectral data

Cat #	Size	Price	Save
394405	25 µg	¥29,920
394406	100 µg	¥76,340

Description

CD44 isoform 9, also known as CD44v9, is a CD44 isofom consisting of exons 1-5, 13 (v9) and 15 to 19, product of alternative splicing. CD44v9 is expressed by cancer stem cells, epithelial-type carcinomas, subsets of monocytes and subsets of lymphocytes; have a role in tumor initiation, maintenance and metastasis of cancer cells. CD44v9 interacts with the glutamate-cystine transporter xCT, increasing reduced glutathione and contributing to the ROS resistance of cancer cells, and is a potential predictive marker for recurrence of some cancers and a potential therapeutic target.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Human CD44v8-10 transfected cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: IHC-P - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue sections³.
RRID: AB_2783305 (BioLegend Cat. No. 394405)
AB_2783305 (BioLegend Cat. No. 394406)

Antigen Details

Distribution: Cancer stem cells, epithelial-type carcinomas, subset of monocytes, subset of lymphocytes.
Function: Role in tumor initiation and maintenance and metastasis of cancer cells, Interacts with the glutamate-cystine transporter xCT, increasing reduced glutathione and contributing to the ROS resistance of cancer cells.
Cell Type: Epithelial cells, Lymphocytes, Monocytes, Neutrophils
Biology Area: Cancer Biomarkers, Cell Biology, Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: Hagiwara M, et al. 2018. BMC Cancer. 18:113

Matsumoto T, et al. 2017. Oncogenesis. 6:397

Bertaux-Skeirik N, et al. 2017. J. Pathol. 242:463

Kodama H, et al. 2017. Br. J. Cancer. 116:186
Gene ID: 960 View all products for this Gene ID
UniProt: View information about CD44v9 on UniProt.org

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Related Protocols

Immunohistochemistry Protocol for Paraffin-Embedded Sections

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Other Formats

View All CD44v9 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human CD44 variant 9 (CD44v9)	RV3	FC,IHC-P,IHC-F
Alexa Fluor® 594 anti-human CD44 variant 9 (CD44v9)	RV3	IHC-P
PE anti-human CD44 variant 9 (CD44v9)	RV3	FC
Alexa Fluor® 647 anti-human CD44 variant 9 (CD44v9)	RV3	FC,IHC-P

Certificate of Analysis

Go To Top Version: 2 Revision Date: 02/22/2023

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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