IHC staining of Alexa Fluor® 594 anti-MECP2 antibody (clone N227/21) on formalin-fixed paraffin-embedded mouse hippocampus tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. Nuclei were counterstained with DAPI, and the slides were mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 10X objective. Scale Bar: 100 µm
IHC staining of Alexa Fluor® 594 anti-MECP2 antibody (clone N227/21) on formalin-fixed paraffin-embedded mouse hippocampus tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. Nuclei were counterstained with DAPI, and the slides were mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 10X objective. Scale Bar: 100 µm
IHC staining of Alexa Fluor® 594 anti-MECP2 antibody (clone N227/21) on formalin-fixed paraffin-embedded rat hippocampus tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. Nuclei were counterstained with DAPI, and the slides were mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 10X objective. Scale Bar: 100 µm
IHC staining of Alexa Fluor® 594 anti-MECP2 antibody (clone N227/21) on formalin-fixed paraffin-embedded mouse thalamus tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. Nuclei were counterstained with DAPI, and the slides were mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scale Bar: 50 µm
IHC staining of Alexa Fluor® 594 anti-MECP2 antibody (clone N227/21) on formalin-fixed paraffin-embedded rat hippocampus tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. Nuclei were counterstained with DAPI, and the slides were mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scale Bar: 50 µm
Methyl CpG binding protein (MECP2), also known as autism susceptibility to X-linked 2 (AUTSX3), is a nuclear protein crucial for normal neuronal function that binds to methylated DNA, forming complexes with histone deacteylases and turning off gene expression. MECP2 expression is an X-linked trait. Loss of MECP2 expression causes Rett Syndrome (also known as RTS, RTT, or RS) a neurological disorder which causes decelerated growth of hands, feet, and the head.
Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
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