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Alexa Fluor® 594 anti-Tubulin Beta 3 (TUBB3) Antibody

Pricing & Availability
Clone
AA10 (See other available formats)
Regulatory Status
RUO
Other Names
β-3 Tubulin, Neuronal class III beta-tubulin, β3-tub
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
A_AA10_AF594_TUBB3_Antibody_1_IF_032315
Day-three cultured postnatal C57BL/6 mouse brain cells were fixed with 1% paraformaldehyde (PFA) for ten minutes, permeabilized with 0.5 % Triton X-100 for ten minutes, and blocked with 5% FBS for 30 minutes. Then the cells were stained with 5 µg/mL of Alexa Fluor® 594 anti-Tubulin Beta 3 (TUBB3) (Clone AA10) (shown in red) in blocking buffer, overnight at 4°C, followed by staining with GFAP (shown in green) at room temperature for two hours. Nuclei were counterstained with DAPI (blue). The image was captured with 40X objective.
  • A_AA10_AF594_TUBB3_Antibody_1_IF_032315
    Day-three cultured postnatal C57BL/6 mouse brain cells were fixed with 1% paraformaldehyde (PFA) for ten minutes, permeabilized with 0.5 % Triton X-100 for ten minutes, and blocked with 5% FBS for 30 minutes. Then the cells were stained with 5 µg/mL of Alexa Fluor® 594 anti-Tubulin Beta 3 (TUBB3) (Clone AA10) (shown in red) in blocking buffer, overnight at 4°C, followed by staining with GFAP (shown in green) at room temperature for two hours. Nuclei were counterstained with DAPI (blue). The image was captured with 40X objective.
  • B_AA10_AF594_TUBB3_Antibody_2_IHCF_092016
    C57BL/6 mouse frozen brain tissue was fixed with 4% paraformaldehyde (PFA) for ten minutes, permeabilized with 0.5 % Triton X-100 for ten minutes, and blocked with 5% FBS for 1 hour. Then the tissue was stained with 1.25 µg/mL of Alexa Fluor® 594 anti-Tubulin Beta 3 (TUBB3) (Clone AA10) (shown in red) and 5 µg/mL of Alexa Fluor® 488 anti-GFAP (Clone 2E1.E9) (shown in green) in blocking buffer, overnight at 4°C. Nuclei were counterstained with DAPI (blue). The image was captured with 10X objective.
  • C_AA10_AF594_TUBB3_Antibody__IHCF_071917
    C57BL/6 mouse frozen brain tissue was fixed with 4% paraformaldehyde (PFA) for ten minutes, permeabilized with 0.5 % Triton X-100 for ten minutes, and blocked with 5% FBS for 30 minutes. Then the tissue was stained with 1.25 µg/mL of Alexa Fluor® 594 anti-Tubulin Beta 3 (TUBB3) (Clone AA10) (red) in blocking buffer, overnight at 4°C. Nuclei were counterstained with DAPI (blue). The image was captured with 10X objective.
  • D_AA10_AF594_TUBB3_Antibody_4_IHCP_091117
    Human paraffin-embedded cerebellum tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Citrate Buffered 1X (1.0M, pH6.0) at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of Alexa Fluor® 647 anti-GFAP Antibody (Clone 2E1.E9, green) and Alexa Fluor® 594 anti-Tubulin Beta 3 (TUBB3) Antibody (Clone AA10, red) antibody overnight at 4°C. Nuclei were counterstained with DAPI (blue). The image was scanned with a 10X objective and stitched with MetaMorph® software.
  • E_AA10_AF594_TUBB3_Antibody_5_IHCP_091117
    Human paraffin-embedded cerebellum tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Citrate Buffered 1X (1.0M, pH6.0) at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of Alexa Fluor® 647 anti-GFAP Antibody (Clone 2E1.E9, blue) and Alexa Fluor® 594 anti-Tubulin Beta 3 (TUBB3) Antibody (Clone AA10, red) antibody overnight at 4°C. Nuclei were counterstained with DAPI (green). The image was scanned with a 10X objective and stitched with MetaMorph® software.
  • F_4_40X_cere_2019-04-26T15-52-43235
    Formalin-fixed, 300 micron-thick mouse brain (cerebellum) section was blocked, permeabilized and stained overnight with Tubulin Beta 3 (TUBB3)(clone AA10) Alexa Fluor® 594 (green) and MAP2 (clone SMI 52) Alexa Fluor® 647 (magenta) both at 5 µg/mL, optically cleared, and analyzed on a confocal microscope. Watch the video.
Compare all formats See Alexa Fluor® 594 spectral data See high resolution IF/IHC-F/IHC-P data...
Cat # Size Price Save
657408 100 µg ¥84,040
Description

Tubulin is the main component of microtubules. In adults, tubulin beta 3 (TUBB3) is primarily expressed in neurons and is commonly used as a neuronal marker. It plays an important role in neuronal cell proliferation and differentiation. Mutations in this gene cause congenital fibrosis of the type 3 extraocular muscles. Tubulin beta 3 (TUBB3) is also found in a wide range of tumors. Studies indicate that it is a predictive and prognostic marker in various tumors.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse, Rat, Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-F - Quality tested
ICC, IHC-P, 3D IHC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemical staining on frozen tissue sections, the suggested use is 0.6 - 5 µg/mL. For immunocytochemistry microscopy, a concentration range of 2.5 - 5.0 μg/mL is recommended. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, the suggested use of this reagent is 5.0 - 10 µg per mL. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Application Notes

Additional reported application (for relevant formats) include: spatial biology (IBEX)1,2.

Application References

(PubMed link indicates BioLegend citation)
  1. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  2. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Metzner K, et al. 2022. Front Cell Dev Biol. 10:965382. PubMed
  2. Wang L, et al. 2023. iScience. 26:106764. PubMed
  3. Albanese A, et al. 2020. Sci Rep. 10:21487. PubMed
RRID
AB_2565285 (BioLegend Cat. No. 657408)

Antigen Details

Structure
450 amino acids with predicted molecular weight of 50 kD
Distribution

Cytosol

Function
Plays important roles in neuronal cell proliferation and differentiation
Interaction
Alpha tubulin, kinesin and dynein
Cell Type
Mature Neurons
Biology Area
Cell Biology, Cell Cycle/DNA Replication, Cell Motility/Cytoskeleton/Structure, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
Microtubules
Antigen References

1. Katsetos CD, et al. 2003. J. Child Neurol. 18:851.
2. Mobarakeh ZT, et al. 2012. Cell Biol. Int. Rep. (2010) 19:e00015.
3. Locher H, et al. 2013. Differentiation. 85:173.
4. Karki R, et al. 2013. Expert Opin. Ther. Targets. 17:461.
5. Mariani M, et al. 2011. Curr. Mol. Med. 11:726.
6. Koh Y, et al. 2009. Ann. Oncol. 20:1414.

Gene ID
10381 View all products for this Gene ID
UniProt
View information about Tubulin beta-3 on UniProt.org
Go To Top Version: 5    Revision Date: 09/13/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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