- Clone
- W16220A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Vim, CTRCT30, HEL 113, Epididymis Luminal Protein 113
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![W16220A_A594_Vimentin_Antibody_020618 W16220A_A594_Vimentin_Antibody_020618](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/W16220A_A594_Vimentin_Antibody_020618.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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NIH3T3 cells were fixed with 4% paraformaldehyde (PFA) for 10 minutes, permeabilized with 0.5% Triton X-100 for 3 minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained overnight at 4 degrees with (A) 1: 250 diluted (2 µg/ml) Alexa Fluor® 594 Rat IgG2b (Cat. No. 400661) or with Alexa Fluor® 594 anti-Vimentin antibody (red) at 1: 250 (2 µg/ml, Figure B) and 1: 1000 (0.5 µg/ml, Figure C) dilution. Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
Cat # | Size | Price | Save |
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699303 | 25 µg | ¥29,920 | |
699304 | 100 µg | ¥76,340 |
Vimentin are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is a widely expressed and highly conserved 54 kD protein that is constitutively expressed in mesenchymal cells, endothelial cells lining blood vessels, renal tubular cells, macrophages, neutrophils, fibroblasts, and leukocytes1,2. Vimentin is used as a marker of mesenchymal cells to distinguish them from epithelial cells3. Increased vimentin expression is frequently used as an EMT marker in cancer4. Autoantibodies to vimentin are commonly found in patients with autoimmune diseases such as Lupus5 and rheumatoid arthritis6, and also found after transplantation7.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Partial mouse Vimentin (224-446 a.a.) recombinant protein expressed in E. coli.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 0.5 - 2.0 μg/ml (1:250 - 1:1000) is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Application Notes
-
This antibody does not cross-react with human (in-house tested).
May be weakly reactive with human Vimentin by WB. - RRID
-
AB_2728517 (BioLegend Cat. No. 699303)
AB_2728518 (BioLegend Cat. No. 699304)
Antigen Details
- Structure
- 466 amino acids with a predicted molecular weight of 54 kD.
- Distribution
-
Cytoplasm.
- Function
- Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
- Interaction
- HCV core protein, LGSN, SYNM, PLEC, SLC6A4, STK33, LARP6, RAB8B, TOR1A, TOR1AIP1, and BCAS3.
- Biology Area
- Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- Intermediate Filaments
- Antigen References
-
1. Kidd ME, et al. 2014. Am J Respir Cell Mol Biol. 50:1.
2. Fuchs E, et al. 1994. Annu Rev Biochem. 63:345.
3. Zeisberg M, et al. 2009. J Clin Invest. 119:1429.
4. Scanlon CS, et al. 2013. J Dent Res. 92:114.
5. Thebault S, et al. 2002. J Immunol. 169:4046.
6. Vossenaar ER, et al. 2004. Arthritis Res Ther. 6:R142.
7. Rose ML. 2013. Hum Immunol. 74:1459. - Gene ID
- 22352 View all products for this Gene ID
- UniProt
- View information about Vimentin on UniProt.org
Related FAQs
Other Formats
View All Vimentin Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-Vimentin | W16220A | WB,ICC,ICFC |
Alexa Fluor® 594 anti-Vimentin | W16220A | ICC |
Alexa Fluor® 488 anti-Vimentin Antibody | W16220A | ICC,ICFC |
Alexa Fluor® 647 anti-Vimentin | W16220A | ICC,ICFC,SB |
PE anti-Vimentin | W16220A | ICFC |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-Vimentin
Total cell lysate (15 µg protein) from A20 (negative control... NIH3T3 cells were fixed with 4% paraformaldehyde (PFA) for ... A20 cells (negative control, open histogram) or EL-4 cells (... -
Alexa Fluor® 594 anti-Vimentin
NIH3T3 cells were fixed with 4% paraformaldehyde (PFA) for 1... -
Alexa Fluor® 488 anti-Vimentin Antibody
NIH/3T3 cells were fixed with 4% paraformaldehyde for 10 min... EL4 cells (filled histogram, positive control) and A20 cells... -
Alexa Fluor® 647 anti-Vimentin
NIH/3T3 cells were fixed with 4% paraformaldehyde for 10 min... EL-4 cells (positive target) were fixed with fixation buffer... A20 cells (negative target) were fixed with fixation buffer ... -
PE anti-Vimentin
EL4 cells (positive control) (filled histogram) and A20 cell...
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