- Clone
- A20145B (See other available formats)
- Regulatory Status
- RUO
- Other Names
- HER2/erbB2, tyrosine kinase cell surface receptor HER2, oncogene ERBB2, oncogene NGL, neuroblastoma- or glioblastoma-derived, HER-2
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Save |
---|---|---|---|
616153 | 25 tests | ¥30,800 | |
616154 | 100 tests | ¥70,400 |
CD340 is the protein HER2, a member of the epidermal growth factor receptor (EGFR) family of cell membrane tyrosine kinases, and is encoded by the gene ErbB2. Unlike other EGFR family members, CD340 contains no ligand binding domain and has no known ligands. Instead, this protein interacts with other EGF receptor family members to form a heterodimer, stabilizing ligand binding, and enhancing kinase-mediated downstream signaling. CD340 has been shown to be involved in embryonic development and cancer progression; this protein is amplified in adenocarcinoma of the lung, some breast cancers, glioblastoma, gastric cancer, and ovarian carcinoma, where it functions as an oncogene. Overexpression of erbB2 in breast cancers has been shown to confer Taxol resistance and is a therapeutic target in a number of human cancers.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant fragment of human ErbB2, N-terminus
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
-
Red Laser (633 nm)
- Application Notes
-
This product is not recommended for use in IHC-P.
- RRID
-
AB_3097216 (BioLegend Cat. No. 616153)
AB_3097216 (BioLegend Cat. No. 616154)
Antigen Details
- Structure
- CD340 is a 1,255 amino acid protein with a predicted molecular weight of 137.9 kDa.
- Distribution
-
Expressed on the cell surface of many tumor cells including some breast cancers, lung adenocarcinoma, gastric cancer, ovarian cancer, glioblastoma, some c-ALL blasts and bone marrow MSCs.
- Function
- Contains no ligand binding domain and interacts with other EGF receptor family members to form a heterodimer, stabilize ligand binding, and enhance kinase-mediated downstream signaling. Involved in embryonic development and cancer progression.
- Interaction
- CD340 interacts with a large number of proteins, including SHC1, EGF receptor, neuroregulin 1, c-Src, integrin β4, Grb2, Grb7, SOS1, caveolin-1, JAK2, PAK1, FAK, and β-catenin, among others.
- Ligand/Receptor
- Despite being a member of the epidermal growth factor receptor (EGFR) family, no ligand of CD340 has been identified.
- Antigen References
-
- Akiyama T, et al. 1986. Science. 232:1644-6.
- Bargmann CI, et al. 1986. Nature. 319:226-30.
- Pegram MD, et al. 1997. Oncogene. 15:537-47.
- Slamon DJ, et al. 1989. Science. 244:707-12.
- Leone F, et al. 2003. J Leukocyte Biol. 74:593-601.
- Gene ID
- 2064 View all products for this Gene ID
- UniProt
- View information about CD340 on UniProt.org
Related Pages & Pathways
Pages
Other Formats
View All CD340 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-CD340 (ErbB2/HER-2) | A20145B | ICC,FC |
Alexa Fluor® 647 anti-CD340 (ErbB2/HER-2) | A20145B | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-CD340 (ErbB2/HER-2)
SK-BR-3 cells were fixed with 4% Fixation Buffer (Cat. No. 4... Human breast cancer cell line (SKBR3) stained with purified ... -
Alexa Fluor® 647 anti-CD340 (ErbB2/HER-2)
Human breast cancer cell line (SK-BR-3) was stained with ant...
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