Alexa Fluor® 647 anti-FOXA1 Antibody, FOXA1, 2F83

Clone: 2F83 (See other available formats)
Regulatory Status: RUO
Other Names: Forkhead box protein A1, HNF3α, Hepatic nuclear factor 3 α
Isotype: Mouse IgG1, κ
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Product Citations: publications

MCF-7 cells were treated with True-Nuclear™ Transcription Factor Buffer Set then stained with anti-FOXA1 (clone 2F83) Alexa Fluor® 647 (filled histogram) or Mouse IgG1, κ Alexa Fluor® 647 isotype control (open histogram).

Cat #	Size	Price	Save
612003	25 µg	¥38,720
612004	100 µg	¥96,800

Description

FOXA1 is a transcriptional activator that is required for tissue-specific differentiation during embryonic development. It also functions as a coactivator with nuclear hormone receptors, including ESR1, and has been shown to play a major role in driving estrogen receptor positive breast cancers.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Full length recombinant human FOXA1
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is ≤ 5 µl per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

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Excitation Laser: Red Laser (633 nm)
Application Notes: When using this clone for western blot, we highly recommend using lysate that has not been subjected to any freeze-thaw cycles.
Additional Product Notes: NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.
RRID: AB_2801117 (BioLegend Cat. No. 612003)
AB_2801117 (BioLegend Cat. No. 612004)

Antigen Details

Structure: FOXA1 is a 472 amino acid protein with a predicted molecular weight of 49 kD.
Distribution: Nucleus/Early endoderm derived cells
Function: Transcriptional activator
Interaction: FOXA2, NKX2-1, Histones H3 and H4
Biology Area: Cell Biology, Transcription Factors
Molecular Family: Nuclear Markers
Gene ID: 3169 View all products for this Gene ID
UniProt: View information about FOXA1 on UniProt.org

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Other Formats

View All FOXA1 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-FOXA1	2F83	WB,ICC,IHC-P
Alexa Fluor® 647 anti-FOXA1	2F83	ICFC

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 04/05/2019

For Research Use Only. Not for diagnostic or therapeutic use.

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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