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Alexa Fluor® 647 anti-HA.11 Epitope Tag Antibody

Pricing & Availability
Clone
16B12 (See other available formats)
Regulatory Status
RUO
Other Names
HA epitope tag, HA1, HA2, hemagglutinin, Hemagglutinin HA1 chain, Hemagglutinin HA2 chain, YPYDVPDYA, Hemagglutinin tag
Isotype
Mouse IgG1, κ
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Product Citations
12 publications
A_16B12_AlexaFluor647_HA11_Antibody_071923
Transfected RBL1 cells expressing the HA tag on the cell surface were stained with anti-HA.11 Epitope Tag (clone 16B12) Alexa Fluor® 647 (filled histogram) or mouse IgG1, κ Alexa Fluor® 647 isotype control (open histogram).
  • A_16B12_AlexaFluor647_HA11_Antibody_071923
    Transfected RBL1 cells expressing the HA tag on the cell surface were stained with anti-HA.11 Epitope Tag (clone 16B12) Alexa Fluor® 647 (filled histogram) or mouse IgG1, κ Alexa Fluor® 647 isotype control (open histogram).
  • B_16B12_A647_HA11-Epitope-Tag_Antibody_102720
    HA tag stably transfected CHO cells were fixed with ice cold methanol for five minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 1 µg/ml Alexa Fluor® 647 conjugated (red) anti-HA.11 Epitope Tag antibody (clone 16B12 ) in blocking buffer for two hours at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
Compare all formats See Alexa Fluor® 647 spectral data
Cat # Size Price Save
682404 100 µg ¥94,380
Description

The HA tag (hemagglutinin) is an amino acid sequence derived from the human influenza hemagglutinin surface glycoprotein, corresponding to amino acids 98-106.  It is commonly used as a tag to facilitate detection, isolation, and purification of proteins. The full amino acid sequence is: YPYDVPDYA.

Product Details
Technical data sheet

Product Details

Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Monoclonal antibody HA.11 was raised against the twelve amino acid peptide CYPYDVPDYASL.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. For immunocytochemistry, a concentration range of 0.1-10 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional tested and reported applications of the 16B12 clone for the relevant formats include: western blot (WB), immunocytochemistry (ICC), immunoprecipitation (IP), and flow cytometry (FC).

*Our Posi-Tag Control Protein (931301) can be used as a helpful positive control for this antibody.

This second-generation HA antibody is an excellent substitute for the 12CA5 monoclonal antibody. The HA.11 antibody recognizes the influenza hemagglutinin epitope (YPYDVPDYA) which has been used extensively as a general epitope tag in expression vectors. The extreme specificity of the antibody allows unambiguous identification and quantitative analysis of the tagged protein. The HA.11 antibody recognizes HA epitopes located in the middle of protein sequences as well as at the N- or C-terminus.

Application References

(PubMed link indicates BioLegend citation)
  1. Kim JY, et al. 2003. J Neurosci. 23:5561. (IP, WB)
  2. Helliwell SB, et al. 2001. J Cell Biol. 153:649. (WB)
  3. Bennett BD, et al. 2000. J Biol Chem. 275:37712. (IF, IP, WB)
  4. Royer Y, et al. 2005. J. Biol. Chem. 29:27251. (FC)
  5. Smith BA, et al. 2012. Genes Cancer. 3:550. (IHC) PubMed
  6. Hogarth C, et al. 2015. Biol Reprod. 93:19. PubMed
  7. Görtz D, et al. 2015. Sci Rep. 5:14685. PubMed
  8. Wilson C, et al. 2015. PLoS One. 10:0139579. PubMed
  9. Smith B, et al. 2012. Genes Cancer. 3:550-563. PubMed
  10. Liu Z, et al. 2016. Nature. 530:98-102. PubMed
  11. Thoms M, et al. 2016. Nucleic Acids Res. 44:926-39. PubMed
  12. Kim Y, et al. 2016. Nat Commun. 7:10347. PubMed
See More
Product Citations
  1. Bielczyk-Maczynska E, et al. 2022. Nat Commun. 13:7408. PubMed
  2. Pymm P, et al. 2022. iScience. 25:105259. PubMed
  3. Willcox CR, et al. 2020. Immunity. 51(5):813-825.e4.. PubMed
  4. Huang P, et al. 2018. Cell. 174:312. PubMed
  5. Pantazis A, et al. 2020. J Physiol. 598:5245. PubMed
  6. Cattin-Ortolá J, et al. 2021. Nat Commun. 12:5333. PubMed
  7. Chai Q, et al. 2021. Cell Reports. 36(6):109514. PubMed
  8. Jandke A, et al. 2020. Nat Commun. 3.075694444. PubMed
  9. Jin SW, et al. 2019. Cell Rep. 29:1449. PubMed
  10. Botzolakis E, et al. 2016. J Biol Chem. 291: 20440 - 20461. PubMed
  11. Mitchell LA, et al. 2019. Oncotarget. 10:2252. PubMed
  12. Smith SE, et al. 2019. J Virol. 93:. PubMed
RRID
AB_2566616 (BioLegend Cat. No. 682404)

Antigen Details

Biology Area
Cell Biology
Gene ID
NA
UniProt
View information about HA.11 on UniProt.org

Related FAQs

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Go To Top Version: 4    Revision Date: 07/19/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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