Alexa Fluor 647 anti-human Aiolos Antibody anti-Aiolos

Clone: 16D9C97 (See other available formats)
Regulatory Status: RUO
Other Names: IKZF3, AIO, ZNFN/A3
Isotype: Mouse IgG1, κ
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Product Citations: publications

16D9C97_A647_Aiolos_Antibody_1_033116 — Human peripheral blood lymphocytes were stained with CD19 Alexa Fluor® 488, treated with the True-Nuclear™ Transcription Factor Buffer Set, and stained with Aiolos (clone 16D9C97) Alexa Fluor® 647 (top) or mouse IgG1, κ Alexa Fluor® 647 isotype control (bottom).

Compare all formats See Alexa Fluor® 647 spectral data

Cat #	Size	Price	Save
371105	25 tests	¥34,980
371106	100 tests	¥86,460

Description

Aiolos, also known as Ikaros family zinc finger 3 (IKZF3), is a 58kD zinc finger protein involved in hematopoetic-specific transcriptional activation. Aiolos is involved in regulation of gene expression in B lymphocytes through chromatin remodeling and formation of homodimers and heterodimers with other members of the Ikaros family. Deletion of Aiolos has been reported in pre-B-acute lymphocytic leukemia (B-ALL).

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Partial Aiolos recombinant protein (1-130aa) expressed in E. Coli.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser: Red Laser (633 nm)
Application Notes: NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.

This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).
RRID: AB_2572171 (BioLegend Cat. No. 371105)
AB_2572171 (BioLegend Cat. No. 371106)

Antigen Details

Structure: 58kD, zinc finger protein.
Distribution: T and B lymphocytes.
Function: Hematopoetic-specific transcriptional activator and chromatin remodeler.
Interaction: Forms heterodimers with Ikaros family members IKZF4 and IKZF5.
Cell Type: T cells, B cells
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Chromatin Remodeling/Epigenetics, Immunology, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. Zhuang Y, et al. 2015. Oncol. Rep. 33:1319.
2. Ma S, et al. 2008. Blood. 111:1396.
3. Billot K, et al. 2010. Leuk. Res. 34:289.
4. Morgan B, et al. 2004. EMBO J. 16:2004.
5. Wang JH, et al. 1998. Immunity. 9:543-53.
6. Hosokawa Y, et al. 1999. Genomics. 61:326.
Gene ID: 22806 View all products for this Gene ID
UniProt: View information about Aiolos on UniProt.org

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Other Formats

View All Aiolos Reagents Request Custom Conjugation

Description	Clone	Applications
PE anti-human Aiolos	16D9C97	ICFC
Purified anti-human Aiolos	16D9C97	ICFC
Alexa Fluor® 647 anti-human Aiolos	16D9C97	ICFC

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 03/31/2016

For Research Use Only. Not for diagnostic or therapeutic use.

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