Alexa Fluor® 647 anti-human CD159a (NKG2A) Antibody, CD159a, S19004C

Clone: S19004C (See other available formats)
Regulatory Status: RUO
Other Names: Killer Cell Lectin Like Receptor C1, KLRC1, NK Cell Receptor A
Isotype: Mouse IgG1, κ
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Product Citations: publications

S19004C_A647_CD159a_Antibody_103020 — Human peripheral blood lymphocytes were stained with CD3 FITC and CD159a (NKG2A) (clone S19004C) Alexa Fluor® 647 (left) or mouse IgG1, κ Alexa Fluor® 647 isotype control (right).

Compare all formats See Alexa Fluor® 647 spectral data

Cat #	Size	Price	Save
375105	25 tests	¥33,660
375106	100 tests	¥87,780

Description

CD159a, also known as NKG2A or KLRC1, is a type II transmembrane protein. It belongs to the killer cell lectin-like receptor family also known as the NKG2 family. It is expressed on NK and NKT cells and T cell subsets. NKG2A is an inhibitory NK cell receptor and it is expressed as a heterodimer with CD94. In human, NKG2A/CD94 complex interacts HLA-E on target cells and inhibits T cells and NK cell effector functions. Recent studies showed that NKG2A blockade enhances the anti-tumor effect of NK cell and CD8⁺ T cells.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human NKG2A/CD94-transfectants
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser: Red Laser (633 nm)
Application Notes: Clone S19004C does not cross-react with mouse CD159a.
RRID: AB_2890806 (BioLegend Cat. No. 375105)
AB_2890806 (BioLegend Cat. No. 375106)

Antigen Details

Structure: Type II transmembrane proteins of the C-type lectin superfamily
Distribution: NK cells, NKT cells, T cell subsets
Function: Inhibiting NK cell receptor
Ligand/Receptor: HLA-E
Cell Type: Lymphocytes, NK cells, NKT cells, T cells
Biology Area: Immuno-Oncology, Immunology
Molecular Family: Immune Checkpoint Receptors
Antigen References: Brooks AG, et al. 1999. J Immunol. 162:305-13.

Kaiser BK, et al. 2008. Proc Natl Acad Sci U S A. 105: 6696-701.

Braud VM, et al. 2003. Trends Immunol. 24:162-4.

Kamiya T, et al. 2019. J Clin Invest. 129:2094.

André P, et al. 2018. Cell. 175:1731.
Gene ID: 3821 View all products for this Gene ID
UniProt: View information about CD159a on UniProt.org

Documentation

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Cell Surface Flow Cytometry Staining Protocol

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Pathways

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Other Formats

View All CD159a Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human CD159a (NKG2A)	S19004C	FC
PE anti-human CD159a (NKG2A)	S19004C	FC
APC anti-human CD159a (NKG2A) Antibody	S19004C	FC
Pacific Blue™ anti-human CD159a (NKG2A) Antibody	S19004C	FC
PE/Cyanine7 anti-human CD159a (NKG2A) Antibody	S19004C	FC
Alexa Fluor® 647 anti-human CD159a (NKG2A) Antibody	S19004C	FC
PE/Cyanine5 anti-human CD159a (NKG2A)	S19004C	FC
APC/Fire™ 750 anti-human CD159a (NKG2A)	S19004C	FC
Alexa Fluor® 700 anti-human CD159a (NKG2A) Antibody	S19004C	FC
PE/Dazzle™ 594 anti-human CD159a (NKG2A) Antibody	S19004C	FC
Biotin anti-human CD159a (NKG2A) Antibody	S19004C	FC
Alexa Fluor® 488 anti-human CD159a (NKG2A)	S19004C	FC
PerCP/Cyanine5.5 anti-human CD159a (NKG2A)	S19004C	FC
FITC anti-human CD159a (NKG2A)	S19004C	FC
TotalSeq™-C1374 anti-human CD159a (NKG2A)	S19004C	PG
TotalSeq™-D1374 anti-human CD159a (NKG2A)	S19004C	PG
TotalSeq™-A1374 anti-human CD159a (NKG2A)	S19004C	PG
APC/Fire™ 810 anti-human CD159a (NKG2A)	S19004C	FC
Brilliant Violet 421™ anti-human CD159a (NKG2A)	S19004C	FC
Brilliant Violet 605™ anti-human CD159a (NKG2A)	S19004C	FC
Brilliant Violet 785™ anti-human CD159a (NKG2A)	S19004C	FC
TotalSeq™-B1374 anti-human CD159a (NKG2A)	S19004C	PG
PerCP/Fire™ 806 anti-human CD159a (NKG2A)	S19004C	FC
PerCP/Fire™ 780 anti-human CD159a (NKG2A)	S19004C	FC
PE/Fire™ 810 anti-human CD159a (NKG2A)	S19004C	FC
APC/Cyanine7 anti-human CD159a (NKG2A)	S19004C	FC
Brilliant Violet 650™ anti-human CD159a (NKG2A)	S19004C	FC
Spark Red™ 718 anti-human CD159a (NKG2A) (Flexi-Fluor™)	S19004C	FC
Brilliant Violet 711™ anti-human CD159a (NKG2A)	S19004C	FC

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 10/30/2020

For Research Use Only. Not for diagnostic or therapeutic use.

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