Alexa Fluor 647 anti-human CD185 CXCR5 Antibody anti-CD185

Clone: J252D4 (See other available formats)
Regulatory Status: RUO
Other Names: BLR1, MDR15
Isotype: Mouse IgG1, κ
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J252D4_AF647_CXCR5_Antibody_1_020713 — Human peripheral blood lymphocytes were stained with CD19 FITC and CD185 (CXCR5, clone J252D4) Alexa Fluor® 647 (top) or mouse IgG1, κ Alexa Fluor® 647 isotype control (bottom).

Compare all formats See Alexa Fluor® 647 spectral data

Cat #	Size	Price	Save
356905	25 tests	¥31,020
356906	100 tests	¥80,080

Description

CD185, also known as CXCR5, is a 42 kD G-protein coupled receptor with seven transmembrane regions. CXCR5 is expressed by mature B cells, follicular helper T cells, Burkitt’s lymphoma cells and a subset of neurons, and mediates cell migration to the B cell follicles in the secondary lymphoid organs. The ligand of CXCR5 is CXCL13 (BLC).

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human CXCR5-transfected cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser: Red Laser (633 nm)
RRID: AB_2561815 (BioLegend Cat. No. 356905)
AB_2561815 (BioLegend Cat. No. 356906)

Antigen Details

Structure: G-protein coupled receptor, 7 transmembrane regions, 42 kD
Distribution: Mature B cells, Follicular helper T cells, and Burkitt's lymphoma cells.
Function: Mediates cell migration to the B cell follicles in the secondary lymphoid organs
Ligand/Receptor: CXCL13
Cell Type: B cells, Tfh
Biology Area: Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References: 1. Ma CS, et al. 2012. J. Exp. Med. 209:1241.
2. León B, et al. 2012. Nat. Immunol. 13:681.
3. Crotty S. 2011. Annu. Rev. Immunol. 29:621.
4. Kerfoot SM, et al. 2011. Immunity 34:947.
5. Sáez de Guinoa J, et al. 2011. Blood 118:1560.
Gene ID: 643 View all products for this Gene ID
UniProt: View information about CD185 on UniProt.org

Documentation

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Related Pages & Pathways

Pathways

Related FAQs

Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?: Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.

Other Formats

View All CD185 Reagents Request Custom Conjugation

Description	Clone	Applications
PE anti-human CD185 (CXCR5)	J252D4	FC
APC anti-human CD185 (CXCR5)	J252D4	FC
Purified anti-human CD185 (CXCR5)	J252D4	FC
Alexa Fluor® 647 anti-human CD185 (CXCR5)	J252D4	FC
PerCP/Cyanine5.5 anti-human CD185 (CXCR5)	J252D4	FC
Alexa Fluor® 488 anti-human CD185 (CXCR5)	J252D4	FC
FITC anti-human CD185 (CXCR5)	J252D4	FC
Alexa Fluor® 700 anti-human CD185 (CXCR5)	J252D4	FC
Pacific Blue™ anti-human CD185 (CXCR5)	J252D4	FC
Brilliant Violet 421™ anti-human CD185 (CXCR5)	J252D4	FC
PE/Cyanine7 anti-human CD185 (CXCR5)	J252D4	FC
APC/Cyanine7 anti-human CD185 (CXCR5)	J252D4	FC
PE/Dazzle™ 594 anti-human CD185 (CXCR5)	J252D4	FC
Brilliant Violet 605™ anti-human CD185 (CXCR5)	J252D4	FC
Biotin anti-human CD185 (CXCR5)	J252D4	FC
Brilliant Violet 711™ anti-human CD185 (CXCR5)	J252D4	FC
Brilliant Violet 785™ anti-human CD185 (CXCR5)	J252D4	FC
TotalSeq™-A0144 anti-human CD185 (CXCR5)	J252D4	PG
TotalSeq™-C0144 anti-human CD185 (CXCR5)	J252D4	PG
Brilliant Violet 750™ anti-human CD185 (CXCR5)	J252D4	FC
TotalSeq™-B0144 anti-human CD185 (CXCR5)	J252D4	PG
APC/Fire™ 750 anti-human CD185 (CXCR5)	J252D4	FC
Spark NIR™ 685 anti-human CD185 (CXCR5)	J252D4	FC
TotalSeq™-D0144 anti-human CD185 (CXCR5)	J252D4	PG
PE/Cyanine5 anti-human CD185 (CXCR5)	J252D4	FC
PE/Fire™ 700 anti-human CD185 (CXCR5) Antibody	J252D4	FC
APC/Fire™ 810 anti-human CD185 (CXCR5) Antibody	J252D4	FC
PerCP/Fire™ 806 anti-human CD185 (CXCR5)	J252D4	FC
PerCP/Fire™ 780 anti-human CD185 (CXCR5)	J252D4	FC
Spark Red™ 718 anti-human CD185 (CXCR5)	J252D4	FC
APC anti-human CD185	J252D4	FC
Brilliant Violet 510™ anti-human CD185 (CXCR5)	J252D4	FC
Brilliant Violet 650™ anti-human CD185 (CXCR5)	J252D4	FC

Certificate of Analysis

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Go To Top Version: 2 Revision Date: 06/27/2014

For Research Use Only. Not for diagnostic or therapeutic use.

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