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Alexa Fluor® 647 anti-human LYPD3 Antibody

Pricing & Availability
Clone
11A1 (See other available formats)
Regulatory Status
RUO
Other Names
C4.4A, GPI-Anchored Metastasis-Associated Protein C4.4A Homolog, LY6/PLAUR Domain Containing 3
Isotype
Mouse IgG2b, κ
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Product Citations
publications
A. 11A1_ALEXA-647_LYPD3_IHC-Antibody_010424
IHC staining of Alexa Fluor® 647 anti-human LYPD3 (clone 11A1). A) Positive on formalin-fixed paraffin-embedded skin tissue and B) Negative on formalin-fixed paraffin-embedded brain tissue. Following antigen retrieval using 1X Citrate buffer 1X pH 6.0 (Cat. No. 420902). The tissue was incubated with 10 µg/mL of Alexa Fluor® 647 anti-human LYPD3 (clone 11A1) overnight at 4°C. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 10X objective.
  • A. 11A1_ALEXA-647_LYPD3_IHC-Antibody_010424
    IHC staining of Alexa Fluor® 647 anti-human LYPD3 (clone 11A1). A) Positive on formalin-fixed paraffin-embedded skin tissue and B) Negative on formalin-fixed paraffin-embedded brain tissue. Following antigen retrieval using 1X Citrate buffer 1X pH 6.0 (Cat. No. 420902). The tissue was incubated with 10 µg/mL of Alexa Fluor® 647 anti-human LYPD3 (clone 11A1) overnight at 4°C. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 10X objective.
  • B. 11A1_ALEXA-647_LYPD3_FC_091623
    MCF-7 cells were stained with anti-human LYPD3 (clone 11A1) Alexa Fluor® 647 (filled histogram) or with mouse IgG2b, κ Alexa Fluor® 647 isotype control (open histogram).
Compare all formats See Alexa Fluor® 647 spectral data
Cat # Size Price Save
364505 25 µg ¥52,800
364506 100 µg ¥104,500
Description

C4.4A, also known as Ly6/PLAUR domain containing 3 (LYPD-3), is a GPI-linked protein with structural similarity to the urokinase-type plasminogen activator receptor (uPAR). C4.4A is also expressed in the placenta, skin and urothelium and can be found in suprabrasal layers of stratified squamous epithelium and is upregulated on migrating keratinocytes during wound healing. Human C4.4A mRNA has been detected in cancer cell lines, including melanoma, breast, bladder, and renal cell carcinoma, as well as in tumor tissue samples from malignant melanoma, colorectal cancer (CRC), breast cancer, lung carcinoma, and urothelial tumors. Expressed in placenta, skin and urothelium. Found in suprabasal keratinocytes of chronic wounds. Weak expression is found in esophagus and peripheral blood mononuclear cells.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-P - Quality tested
FC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10.0 µg/mL is suggested. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
RRID
AB_3097239 (BioLegend Cat. No. 364505)
AB_3097239 (BioLegend Cat. No. 364506)

Antigen Details

Distribution

Placenta, skin, urothelium, keratinocytes

Function
Interacting selectively and non-covalently with laminins, glycoproteins that are major constituents of the basement membrane of cells
Interaction
LGALS3 (By similarity). AGR2 and AGR3
Ligand/Receptor
Laminin-1 and laminin-5
Biology Area
Cell Adhesion
Molecular Family
Adhesion Molecules
Antigen References
  1. Smith BA, et al. 2001. Cancer Res. 61:1678-85.
  2. Würfle J, et al. 2001. Gene. 262:35-41.
Gene ID
27076 View all products for this Gene ID
UniProt
View information about LYPD3 on UniProt.org
Go To Top Version: 1    Revision Date: 09/15/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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