Alexa Fluor® 647 anti-mouse CD146 Antibody, CD146, A20078A

Clone: A20078A (See other available formats)
Regulatory Status: RUO
Other Names: S-Endo 1 antigen, MUC18, MCAM, Mel-CAM, A32 antigen
Isotype: Rat IgG2b, κ
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Product Citations: publications

1. A20078A_A647_CD146_Antibody_1_072823 — C57BL/6 mouse splenocytes were stained with anti-mouse NK-1.1 FITC and anti-mouse CD146 (clone A20078A) Alexa Fluor® 647 (left), or rat IgG2b, κ Alexa Fluor® 647 isotype control (right).

Compare all formats See Alexa Fluor® 647 spectral data

Cat #	Size	Price	Save
112005	25 µg	¥29,700
112006	100 µg	¥62,700

Description

CD146, also known as melanoma cell adhesion molecule (MCAM or Mel-CAM), MUC18, S-Endo1, and A32 antigen, is an integral membrane glycoprotein that belongs to the Ig superfamily. CD146 is strongly expressed by murine vascular endothelial cells. It is expressed on about 30% of neutrophils and 60% of NK cells. Unlike in humans, CD146 is undetectable on monocytes, dendritic cells, T cells, NKT cells, B cells, or smooth muscle cells in mouse. It has been reported that an increase in CD146 expression is associated with NK cell maturation. Combined with using CD27 and CD11b staining, CD146 may be an alternative marker to detect final stages of NK cell maturation and define NK cell subsets. CD146+ NK cells were found to be less cytotoxic and to produce less IFN-γ than CD146- NK cells upon stimulation with target cells or activating antibodies. The role of CD146 on NK cell migration has yet to be investigated. The identification of CD146 ligand(s) will be crucial to address this issue.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant protein (val 24-val 563 extracellular domain)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
IHC-F - Verified
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. For immunohistochemistry on frozen tissue sections, a concentration range of 5.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser: Red Laser (633 nm)
RRID: AB_3068233 (BioLegend Cat. No. 112005)
AB_3068233 (BioLegend Cat. No. 112006)

Antigen Details

Structure: CD146 is a transmembrane glycoprotein and belongs to the Ig superfamily of cell adhesion molecules.
Distribution: CD146 is strongly expressed by murine vascular endothelial cells, a subset of NK1.1+ cells, and neutrophils.
Function: CD146 has been reported to be functionally relevant for endothelial cell adhesion, angiogenesis, and NK cell function.
Ligand/Receptor: Unidentified ligand
Cell Type: Endothelial cells, Epithelial cells, Mesenchymal cells, Mesenchymal Stem Cells, T cells
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family: Adhesion Molecules, CD Molecules
Gene ID: 84004 View all products for this Gene ID
UniProt: View information about CD146 on UniProt.org

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Other Formats

View All CD146 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-mouse CD146	A20078A	FC,IHC-F
Alexa Fluor® 488 anti-mouse CD146	A20078A	FC,IHC-F
Alexa Fluor® 594 anti-mouse CD146	A20078A	FC
Alexa Fluor® 647 anti-mouse CD146	A20078A	FC,IHC-F

Certificate of Analysis

Go To Top Version: 1 Revision Date: 07/28/2023

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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