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Alexa Fluor® 647 anti-mouse CD68 Antibody

Pricing & Availability
Clone
FA-11 (See other available formats)
Regulatory Status
RUO
Other Names
Macrosialin
Isotype
Rat IgG2a
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Product Citations
publications
FA-11_A647_021810
Thioglycolate-elicited Balb/c peritoneal macrophages intracelluar stained with FA-11 Alexa Fluor® 647
  • FA-11_A647_021810
    Thioglycolate-elicited Balb/c peritoneal macrophages intracelluar stained with FA-11 Alexa Fluor® 647
Compare all formats See Alexa Fluor® 647 spectral data
Cat # Size Price Save
137003 25 µg ¥32,340
137004 100 µg ¥76,340
Description

Mouse CD68, also known as macrosialin, is an 85-115 kD member of the lysosomal-associated membrane protein (LAMP) family. It is a heavily glycosylated and predominantly intracellular protein, mainly in late endosomes. Macrosialin is the murine homolog to the human macrophage glycoprotein CD68. It is expressed on tissue macrophages, Langerhans cells and at low levels on dendritic cells. Lamp proteins may have functions relating to cell-cell interaction or cell-ligand interaction. The biological function of CD68 is not completely understood.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Purified Con A receptor glycoproteins from the P815 cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
FC - Verified
SB - Community verified
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported (for relevant formats) applications include: immunoprecipitation1, 2, Western Blot1, 2, immunohistochemical staining of frozen sections2 and paraformaldehyde-fixed paraffin-embedded sections3, and spatial biology (IBEX)9,10.

Additional Product Notes

This product has been verified for IHC-P (Immunohistochemistry - formalin-fixed paraffin-embedded tissues) on the NanoString GeoMx® Digital Spatial Profiler. The GeoMx® enables researchers to perform spatial analysis of protein and RNA targets in FFPE and fresh frozen human and mouse samples. For more information about our spatial biology products and the GeoMx® platform, please visit our spatial biology page.

Application References

(PubMed link indicates BioLegend citation)
  1. Silva RP, et al. 1999. Biochem. J. 338:687. (IP, WB)
  2. Rabinowitz SS, et al. 1991. J. Exp. Med. 174:827. (IP, WB, IHC)
  3. Wu J, et al. 2008. P. Natl. Acad. Sci. USA 105:16934. (IHC)
  4. Kayama H, et al. 2012. PNAS. 109:5010. PubMed
  5. Park S, et al. 2013. Biomaterials. 34:598. PubMed
  6. Guiducci C, et al. 2013. J Exp Med. 210:2903. PubMed
  7. McKinstry SU, et al. 2014. J Neurosci. 34:9455. PubMed
  8. Li X, et al. 2015. J Am Heart Assoc. 6:4. PubMed
  9. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  10. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Dolfi B, et al. 2022. Cell Rep. 39:110949. PubMed
  2. Wecker L, et al. 2010. Neuroscience. 12:171. PubMed
  3. Benvie AM, et al. 2024. iScience. 108682:27. PubMed
  4. Carlock C, et al. 2013. Reproduction. 146:491. PubMed
  5. Roufaiel M, et al. 2016. Nat Immunol. 10.1038/ni.3564. PubMed
  6. Nguyen SL, et al. 2021. Sci Rep. 11:4217. PubMed
  7. Zhang C, et al. 2021. Clin Transl Immunology. 10:e1310. PubMed
  8. Nie M, et al. 2016. Cell Death Dis. 7:e2261. PubMed
  9. Montagner M, et al. 2020. Nat Cell Biol. 289:22. PubMed
  10. Neupane AS, et al. 2020. Cell. 183(1):110-125.e11. PubMed
  11. Baasch S, et al. 2021. Cell. . PubMed
  12. Calderon B, et al. 2015. J Exp Med. 212: 1497-1512. PubMed
  13. Johansson A, et al. 2012. Proc Natl Acad Sci U S A. 109:7841. PubMed
  14. Aggarwal N, et al. 2021. Cell Rep. 37:110170. PubMed
RRID
AB_2044001 (BioLegend Cat. No. 137003)
AB_2044001 (BioLegend Cat. No. 137004)

Antigen Details

Structure
A member of the lysosomal-associated membrane protein (lamp) family.
Distribution

Expressed on tissue macrophages, Langerhans cells, and at low levels on dendritic cells.

Function
Involved in cell-cell interaction or cell-ligand interaction, still not completely understood.
Cell Type
Antigen-presenting cells, Dendritic cells, Langerhans cells, Leukocytes, Macrophages
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
Adhesion Molecules, CD Molecules, Innate Immune Signaling
Antigen References

1. Ramprasad MP, et al. 1996. Proc. Natl. Acad. Sci. USA 93:14833.
2. Smith MJ, et al. 1987. J. Cell. Sci. 87:113.

Gene ID
12514 View all products for this Gene ID
UniProt
View information about CD68 on UniProt.org

Related FAQs

If an antibody clone has been previously successfully used in IBEX in one fluorescent format, will other antibody formats work as well?

It’s likely that other fluorophore conjugates to the same antibody clone will also be compatible with IBEX using the same sample fixation procedure. Ultimately a directly conjugated antibody’s utility in fluorescent imaging and IBEX may be specific to the sample and microscope being used in the experiment. Some antibody clone conjugates may perform better than others due to performance differences in non-specific binding, fluorophore brightness, and other biochemical properties unique to that conjugate.

Will antibodies my lab is already using for fluorescent or chromogenic IHC work in IBEX?

Fundamentally, IBEX as a technique that works much in the same way as single antibody panels or single marker IF/IHC. If you’re already successfully using an antibody clone on a sample of interest, it is likely that clone will have utility in IBEX. It is expected some optimization and testing of different antibody fluorophore conjugates will be required to find a suitable format; however, legacy microscopy techniques like chromogenic IHC on fixed or frozen tissue is an excellent place to start looking for useful antibodies.

Are other fluorophores compatible with IBEX?

Over 18 fluorescent formats have been screened for use in IBEX, however, it is likely that other fluorophores are able to be rapidly bleached in IBEX. If a fluorophore format is already suitable for your imaging platform it can be tested for compatibility in IBEX.

The same antibody works in one tissue type but not another. What is happening?

Differences in tissue properties may impact both the ability of an antibody to bind its target specifically and impact the ability of a specific fluorophore conjugate to overcome the background fluorescent signal in a given tissue. Secondary stains, as well as testing multiple fluorescent conjugates of the same clone, may help to troubleshoot challenging targets or tissues. Using a reference control tissue may also give confidence in the specificity of your staining.

How can I be sure the staining I’m seeing in my tissue is real?

In general, best practices for validating an antibody in traditional chromogenic or fluorescent IHC are applicable to IBEX. Please reference the Nature Methods review on antibody based multiplexed imaging for resources on validating antibodies for IBEX.

Go To Top Version: 4    Revision Date: 01/23/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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