- Clone
- W17001A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Eomesodermin, T-box brain protein 2 (TBR-2)
- Isotype
- Rat IgG2b, λ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![W17001A_A647_EOMES_Antibody_071619.png W17001A_A647_EOMES_Antibody_071619.png](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/W17001A_A647_EOMES_Antibody_071619.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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C57BL/6 mouse splenocytes were surface stained with CD8a Alexa Fluor® 488 then treated with True-Nuclear Transcription Factor Buffer Set. Cells were then stained with EOMES (clone W17001A) Alexa Fluor® 647 (left) or Rat IgG2b Alexa Fluor® 647 isotype control (right).
Eomesodermin, or EOMES, is a transcription factor in the T-box family. During embryonic development, EOMES is crucial in regulating trophoblast differentiation, gastrulation, mesoderm delamination, and development of the cerebral cortex. In the immune system, EOMES is involved in the activation, migration, and differentiation of CD8+ T cells. In cooperation with another T-box transcription factor, T-bet, EOMES induces production of IFN-γ and enhances cytotoxic activities of effector CD8+ T cells. EOMES has been shown to be required to maintain long-term memory of CD8+ T cells and is important for homeostasis of memory and effector T cells.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse EOMES recombinant protein (463-707 a.a.) expressed in E. coli.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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ICFC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
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Red Laser (633 nm)
- Application Notes
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W17001A was tested for ICC using 4% PFA-fixed HeLa (negative control) and NK-92 (positive control cells) permeabilized with either Triton X-100 or methanol. Both methods resulted in high non-specific staining in HeLa cells. We therefore don’t recommend W17001A for this application.
- Product Citations
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- RRID
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AB_2814093 (BioLegend Cat. No. 157703)
Antigen Details
- Distribution
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Nucleus
- Antigen References
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- Cooper L, et al. 2018. Plos One. 13(12).
- Pikovskaya O, et al. 2016. J.Immunol. 196(4):1449-54.
- Lupar E, et al. 2015. J.Immunol. 195(10):4742-52.
- Gene ID
- 13813 View all products for this Gene ID
- UniProt
- View information about EOMES on UniProt.org
Related Pages & Pathways
Pages
Other Formats
View All EOMES Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Alexa Fluor® 647 anti-mouse EOMES | W17001A | ICFC |
Purified anti-mouse EOMES | W17001A | ICFC,WB,IP,IHC-P |
PE anti-mouse EOMES Antibody | W17001A | ICFC |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Alexa Fluor® 647 anti-mouse EOMES
C57BL/6 mouse splenocytes were surface stained with CD8a Ale... -
Purified anti-mouse EOMES
C57BL/6 mouse splenocytes were surface stained with CD8a Ale... Whole cell extracts (15 µg total protein) from NK-92 (human ... Whole cell extracts (250 µg total protein) prepared from NK-... IHC staining of purified anti-mouse EOMES (clone W17001A) on... -
PE anti-mouse EOMES Antibody
C57BL/6 mouse splenocytes were stained with anti-mouse CD8a ...
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