Alexa Fluor 647 anti-mouse/human Bcl-6 Antibody anti-Bcl-6

Clone: IG191E/A8 (See other available formats)
Regulatory Status: RUO
Other Names: B-cell lymphoma 6, BCL-5, LAZ3, BCL6A, ZNF51, ZBTB27
Isotype: Mouse IgG1
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Product Citations: publications

IG191E-A8_A647_Bcl6_Antibody_1_102224 — C57BL/6 lymph node cells were immunized with CFA for 12 days, fixed, and permeabilized with BioLegend Nuclear Factor Fixation and Permeabilization Buffer Set, then stained with CD19 FITC, IgD PerCP/Cyanine5.5, and Bcl-6 (clone IG191E/A8) Alexa Fluor® 647 (left) or mouse IgG1, κ Alexa Fluor® 647 isotype control (right). Dot plots are gated on CD19⁺ lymphocytes.

Compare all formats See Alexa Fluor® 647 spectral data

Cat #	Size	Price	Save
648305	25 tests	¥32,340
648306	100 tests	¥89,100

Description

B-cell lymphoma 6 (Bcl-6), is an 80 kD homodimer and member of the BTB-POZ zinc finger family. It consists of one BTB (POZ) domain and six C2H2-type zinc fingers. Bcl-6 is a transcriptional repressor as well as a master regulator of germinal center reaction. On B cells, Bcl-6 induces proliferation, antibody class switching and affinity maturation, while inhibits its differentiation to plasma cells. On T cells, Bcl-6 induces its differentiation to T_FH. This molecule is also expressed in some B cell lymphomas and breast cancer cells.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Plasmid vector containing cDNA of the Bcl-6 gene
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser: Red Laser (633 nm)
Application Notes: This clone is also known as GI191E/A8. Additional reported applications (for the relevant formats) include: immunohistochemical staining of formalin fixed, paraffin embedded tissue sections¹, Western blotting¹, and immunoprecipitation¹.
RRID: AB_2565298 (BioLegend Cat. No. 648305)
AB_2565298 (BioLegend Cat. No. 648306)

Antigen Details

Structure: Member of the BTB-POZ zinc finger family, 80 kD, homodimer, 1 BTB (POZ) domain, 6 C2H2-type zinc fingers
Distribution: Germinal center B cells, follicular helper T cells (TFH), B cell lymphomas, breast cancer cells
Function: Transcription factor, master regulator of germinal center reaction
Interaction: BLIMP-1
Cell Type: B cells, Tfh
Biology Area: Cell Biology, Immunology, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. Ye BH, et al. 1993. Science 262:747.
2. Baron BW, et al. 1993. P. Natl. Acad. Sci. USA 90:5262.
3. Onizuka T, et al. 1995. Blood 86:28.
4. Johnston RJ, et al. 2009. Science 325:1006.
5. Kitano M, et al. 2011. Immunity 34:961.
6. Baumjohann D, et al. 2011. J. Immunol. 187:2089.
7. Tran TH, et al. 2010. Cancer Res. 70:1711.
Gene ID: 604 View all products for this Gene ID 12053 View all products for this Gene ID
UniProt: View information about Bcl-6 on UniProt.org

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Other Formats

View All Bcl-6 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-mouse/human Bcl-6	IG191E/A8	WB,ChIP,IHC-P,IP,SB
PE anti-mouse/human Bcl-6	IG191E/A8	ICFC
Alexa Fluor® 647 anti-mouse/human Bcl-6	IG191E/A8	ICFC
Alexa Fluor® 594 anti-mouse/human Bcl-6	IG191E/A8	IHC-P

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 05/22/2015

For Research Use Only. Not for diagnostic or therapeutic use.

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