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- Clone
- KH116 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- MHC class II
- Isotype
- Mouse (BALB/c) IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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FVB mouse splenocytes stained with KH116 Alexa Fluor® 647
The KH116 antibody reacts with the I-Aq MHC class II alloantigen. These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2q bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins. The KH116 antibody cross-reacts with I-As and I-Av; reactivity with other haplotypes has not been reported.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- B10.Q mouse splenocytes
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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FC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
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Red Laser (633 nm)
- Application Notes
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Additional reported applications (for the relevant formats) include: complement-mediated cytotoxicity1, immunoprecipitation3, and immunohistochemical staining of acetone-fixed frozen sections. Does not cross react with other haplotypes (e.g., a, b, d, f, k, n, p, r, u).
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Application References
(PubMed link indicates BioLegend citation) -
- Hasendrug K, et al. 1987. Immunogenetics 25:136.
- Zuo L, et al. 2002. J. Immunol. 168:2554.
- Nakagawa TY, et al. 1999. Immunity 10:207.
- Podolin PL, et al. 2008. J. Immunol. 180:7989. (FC) PubMed
- Product Citations
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- RRID
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AB_493174 (BioLegend Cat. No. 115208)
Antigen Details
- Structure
- MHC class II
- Distribution
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B cell and activated T cells, APCs of H-2q mice
- Function
- Antigen presentation
- Ligand/Receptor
- CD3/TCR, CD4
- Cell Type
- Antigen-presenting cells, B cells, T cells, Tregs
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- MHC Antigens
- Antigen References
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1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323. - Gene ID
- 14961 View all products for this Gene ID
- UniProt
- View information about I-Aq on UniProt.org
Related Pages & Pathways
Pathways
Related FAQs
Other Formats
View All I-Aq Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Alexa Fluor® 647 anti-mouse I-Aq | KH116 | FC |
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Alexa Fluor® 647 anti-mouse I-Aq
FVB mouse splenocytes stained with KH116 Alexa Fluor® 647
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