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Alexa Fluor® 647 anti-mouse PD-1H (VISTA) Antibody

Pricing & Availability
Clone
MH5A (See other available formats)
Regulatory Status
RUO
Other Names
PD-1 homolog, Dies1, B7-H5
Isotype
Armenian Hamster IgG
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Product Citations
publications
MH5A_A647_PD-1H_Antibody_013018
57BL/6 mouse splenocytes were stained with CD8a PE and PD-1H (clone MH5A) Alexa Fluor® 647(left) or Armenian Hamster IgG Alexa Fluor® 647 isotype control (right).
  • MH5A_A647_PD-1H_Antibody_013018
    57BL/6 mouse splenocytes were stained with CD8a PE and PD-1H (clone MH5A) Alexa Fluor® 647(left) or Armenian Hamster IgG Alexa Fluor® 647 isotype control (right).
Compare all formats See Alexa Fluor® 647 spectral data
Cat # Size Price Save
143721 25 µg ¥34,980
143722 100 µg ¥82,500
Description

PD-1H, also known as VISTA, is a 309 aa type I transmembrane protein, composed of seven exons. PD-1H has one Ig-V like domain, and its sequence is similar to the Ig-V domains of the members of CD28 and B7 families. PD-1H is expressed by a subset of T cells, macrophages, dendritic cells, neutrophils, and NK cells. It has been proposed that PD-1H can be useful to modulate the host immune response to allogeneic transplants. 

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
PD-1H- IgG Fc fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: inhibition of graft vs host disease (GVHD), Western blotting, and immunohistochemical staining of paraffin embedded tissue sections.

Application References

(PubMed link indicates BioLegend citation)
  1. Flies DB, et al. 2011. J. Immunol. 187:1537.
RRID
AB_2728166 (BioLegend Cat. No. 143721)
AB_2728166 (BioLegend Cat. No. 143722)

Antigen Details

Structure
Type I transmembrane protein, member of the CD28 family
Distribution

Macrophages, dendritic cells, neutrophils, NK cells, naïve and activated T cells

Function
Negative regulator of the immune response
Cell Type
Dendritic cells, Macrophages, Neutrophils, NK cells, T cells
Biology Area
Immunology, Inhibitory Molecules
Antigen References

1. Flies DB, et al. 2011. J. Immunol. 187:1537.
2. Wang Li, et al. 2011. J. Exp Med. 208:577.

Gene ID
74048 View all products for this Gene ID
UniProt
View information about PD-1H on UniProt.org
Go To Top Version: 1    Revision Date: 01/30/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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