Alexa Fluor 647 anti-mouse Qa-2 Antibody anti-Qa-2

Clone: 695H1-9-9 (See other available formats)
Regulatory Status: RUO
Other Names: Nonclassical MHC-Ib, H2-Qa2
Isotype: Mouse IgG2a, κ
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695H1-9-9-Alx647_081406 — C57BL/6 mouse splenocytes stained with 695H1-9-9 Alexa Fluor® 647

Compare all formats See Alexa Fluor® 647 spectral data

Cat #	Size	Price	Save
121707	25 µg	¥22,220
121708	100 µg	¥50,380

Description

Mouse Qa-2 is a 40 kD GPI-linked surface protein. It is a member of nonclassical MHC class Ib molecule encoded by Q5, Q6, Q7, Q9, and Q10 genes of the H-2 complex. Qa-2 is a nonpolymorphic molecule primarily expressed on mouse T and B cells. The expression level of Qa-2 on mature T cells is higher than on B cells. Different mouse strains express Qa-2 at varying levels. Qa-2, in association with β2-microglobulin, binds to peptide and plays a role in antigen presentation.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: C3H.SW mouse skin graft and splenocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation².
RRID: AB_571988 (BioLegend Cat. No. 121707)
AB_571988 (BioLegend Cat. No. 121708)

Antigen Details

Structure: GPI-linked protein, associates with β2-microglobulin, 40 kD
Distribution: T and B cells, mature T cells expression higher than B cells
Function: Antigen presentation, promote immune response
Ligand/Receptor: Peptide
Cell Type: B cells, T cells
Biology Area: Immunology
Molecular Family: MHC Antigens
Antigen References: 1. Sharrow SO, et al. 1989. J. Immunol. 142:3495.
2. Mann DW, et al. 1987. J. Immunol. 138:240.
3. Mellor AL, et al.1985. Proc. Natl. Acad. Sci.USA. 82:5920.
4. Tabaczewski P, et al. 1997. J. Immunol. 159:2771.
5. Chiang EY and Stroynowski I. 2005. J. Immunol. 174:5367.
Gene ID: 111458 View all products for this Gene ID
UniProt: View information about Qa-2 on UniProt.org

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Other Formats

View All Qa-2 Reagents Request Custom Conjugation

Description	Clone	Applications
Biotin anti-mouse Qa-2	695H1-9-9	FC
Alexa Fluor® 647 anti-mouse Qa-2	695H1-9-9	FC
FITC anti-mouse Qa-2	695H1-9-9	FC
Purified anti-mouse Qa-2	695H1-9-9	FC
APC anti-mouse Qa-2	695H1-9-9	FC
PE anti-mouse Qa-2	695H1-9-9	FC

Certificate of Analysis

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Go To Top Version: 3 Revision Date: 01/24/2017

For Research Use Only. Not for diagnostic or therapeutic use.

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