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- Clone
- F31-5G3 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- T cell immunoglobulin and mucin domain containing protein-4
- Isotype
- Rat IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Balb/c mouse peritoneal macrophages stained with F31-5G3 Alexa Fluor® 647 and CD11b (M1/70) FITC
TIM4 is a transmembrane protein known as T cell immunoglobulin and mucin domain containing protein-4. It is expressed on antigen-presenting cells and not on T cells.TIM4 binds to TIM1 to promote T cell proliferation by enhancing cell division and reducing apoptosis.TIM4 bind to phosphatidylserine through its FG-CC’ binding cleft in the N-terminal IgV domain to facilitate the clearance of apoptotic cells or bodies.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse TIM4-Ig fusion protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
-
Red Laser (633 nm)
-
Application References
(PubMed link indicates BioLegend citation) -
- Rodriguez-Manzanet R, et al. 2008. J. Immunol. 180:4706
- RRID
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AB_1227797 (BioLegend Cat. No. 129907)
Antigen Details
- Structure
- Transmembrane protein containing immunoglobulin domain and mucin-like domain
- Distribution
-
Unlike the other TIMs, TIM4 is only expressed by antigen-presenting cells, but not by T cells.
- Function
- TIM4 is expressed on antigen-presenting cells. It provides co-stimulation to promote T cell proliferation and survival and has a possible role in clearance of apoptotic cells by binding phosphatidylserine.
- Interaction
- Phosphatiylserine is the ligand of TIM4 and TIM4 is a ligand of TIM1
- Ligand/Receptor
- Phosphatidylserine, TIM1
- Cell Type
- Antigen-presenting cells
- Biology Area
- Immunology
- Antigen References
-
1. Kuchroo VK, et al. 2008. Nat. Rev. Immunol. 8:577
2. Miyanishi M et al. 2007. Nature 450:435
3. Rodriguez-Manzanet R et al. 2008. J. Immunol. 180:4706 - Gene ID
- 276891 View all products for this Gene ID
- UniProt
- View information about Tim-4 on UniProt.org
Related Pages & Pathways
Pathways
Related FAQs
Other Formats
View All Tim-4 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| PE anti-mouse Tim-4 | F31-5G3 | FC |
| Alexa Fluor® 647 anti-mouse Tim-4 | F31-5G3 | FC |
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Compare Data Across All Formats
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PE anti-mouse Tim-4
BALB/c peritoneal macrophages stained with F31-5G3 PE and M1... -
Alexa Fluor® 647 anti-mouse Tim-4
Balb/c mouse peritoneal macrophages stained with F31-5G3 Ale...
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