Alexa Fluor 647 anti-Nanog Antibody anti-Nanog

Clone: 23D2-3C6 (See other available formats)
Regulatory Status: RUO
Other Names: Homeobox Transcription Factor Nanog, Nanog Homeobox, Homeobox Protein NANOG, Homeobox Transcription Factor Nanog-Delta 48
Isotype: Mouse IgG1, κ
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23D2-3C6_A647_Nanog_Antibody_ICFC_093016 — NTERA-2 cells were treated with the True-Nuclear™ Transcription Factor Buffer Set and then intracellularly stained with anti-Nanog (clone 23D2-3C6) Alexa Fluor® 647 (filled histogram) or mouse IgG1, κ Alexa Fluor® 647 isotype control (open histogram).

Compare all formats See Alexa Fluor® 647 spectral data

Cat #	Size	Price	Save
674010	100 µg	¥84,040

Description

Nanog is a transcription factor that plays key roles in the maintenance of pluripotency and self-renewal in embryonic stem cells. Nanog expression is regulated by other transcription factors, which include Sox2 and Oct-4. It also plays a role in epithelial malignancy and is enriched in cancer stem cells. The ablation of nanog is sufficient to reduce the cancer stem cell pool.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Full length human recombinant protein expressed in bacteria
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser: Red Laser (633 nm)
Application Notes: NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.

This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).
RRID: AB_2632605 (BioLegend Cat. No. 674010)

Antigen Details

Structure: 305 amino acids with a predicted molecular weight of approximately 35 kD.
Distribution: Nuclear and cytoplasmic. Expressed by embryonic stem (ES) cells or cancer stem cells. Expression decreases with ES differentiation.
Function: Transcription regulator that plays key roles in the maintenance of pluripotency and self-renewal in embryonic stem cells.
Interaction: Interacts with SMAD1, SALL4, and ZNF281/ZFP281.
Cell Type: Embryonic Stem Cells
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Signal Transduction, Stem Cells, Transcription Factors
Antigen References: 1. Hwang CC, et al. 2014. PLoS One 9:e105676.
2. Torres-Padilla ME, et al. 2014. Development 141:2173.
3. Iv Santaliz-Ruiz LE, et al. 2014. Int. J. Cancer 12:2741.
4. Wang ML, et al. 2013. Onco. Targets Ther. 6:1207.
5. Kim J, et al. 2008. Cell 132:1049.
6. Takahashi K, et al. 2007. Nat. Protoc. 2:3081.
7. Yu J, et al. 2007. Science 318:1917.
Gene ID: 79923 View all products for this Gene ID
UniProt: View information about Nanog on UniProt.org

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Other Formats

View All Nanog Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-Nanog	23D2-3C6	WB,ICFC,ICC
Alexa Fluor® 594 anti-Nanog	23D2-3C6	ICC
Alexa Fluor® 488 anti-Nanog	23D2-3C6	ICC
Alexa Fluor® 647 anti-Nanog	23D2-3C6	ICFC

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 10/03/2016

For Research Use Only. Not for diagnostic or therapeutic use.

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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