Alexa Fluor® 647 anti-NRF2 Antibody, NRF2, W19086B

Clone: W19086B (See other available formats)
Regulatory Status: RUO
Other Names: Erythroid 2 Like 2, NF-E2-Related Factor 2, HEBP1, IMDDHH
Isotype: Rat IgG2a, κ
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Product Citations: publications

W19086B_A647_NRF2_Antibody_110722 — HeLa cells untreated (negative control, open histogram), or treated with 10 µM of MG-132 overnight (positive control, solid histogram) were fixed and permeabilized using True Nuclear Transcription Factor Buffer (Cat No. 424401) and intracellularly stained with Alexa Fluor® 647 anti-NRF2 (clone W19086B), or Alexa Fluor® 647 Rat IgG2a, κ isotype control (open histogram, dashed line)(representative for both untreated and MG-132 treated HeLa cells) (Cat No. 400526).

Compare all formats See Alexa Fluor® 647 spectral data

Cat #	Size	Price	Save
939205	25 tests	¥36,300
939206	100 tests	¥82,500

Description

NRF2 is a transcription factor that plays a critical role in inducing expression of genes required for oxidative stress defense and stress balance. It does so by binding to antioxidant response elements (AREs), which are located upstream of target genes. NRF2 is degraded by ubiquitination of KEAP1 E3 ligase. In a clinical setting, NRF2 is frequently activated in many types of cancers; it drives metabolic adaptation and survival in ROS-rich tumor microenvironments.

Product Details

Technical data sheet

Product Details

Verified Reactivity

Human

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Partial recombinant human NRF2 protein

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser

Red Laser (633 nm)

Application Notes

This clone was tested for ICC on 4% PFA-fixed HeLa cells and permeabilized with either methanol or Triton X-100. Both permeabilization methods were compatible with NRF2 staining.

Clone W19086B is validated to detect NRF2 in mouse NIH/3T3 cells using a Western blot on whole cell lysate.

RRID

AB_2927972 (BioLegend Cat. No. 939205)
AB_2927972 (BioLegend Cat. No. 939206)

Antigen Details

Structure: NRF2 is a 605 amino acid protein with a predicted molecular weight of 67 kD.
Distribution: Ubiquitously expressed/Nucleus
Function: Transcription factor/Redox balance
Biology Area: Cell Biology, Mitochondrial Function, Transcription Factors
Antigen References: M. da Costa R, et al. 2019. Front In Pharm. 10:3389.

Shoemaker A. 2017. Sci Trans Med. 9:420.

Robledinos-Antón N, et al. 2019. Hindawi. 10:1155.

Cuadrado A, et al. 2019. Nat Rev Drug Discov. 18:295-317.
Gene ID: 4780 View all products for this Gene ID
UniProt: View information about NRF2 on UniProt.org

Documentation

Reviews

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Related Protocols

Intracellular Flow Cytometry Staining Protocol

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Other Formats

View All NRF2 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-NRF2	W19086B	WB,ICC,IP,IHC-P,ICFC
PE anti-NRF2	W19086B	ICFC
Alexa Fluor® 647 anti-NRF2	W19086B	ICFC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 10/27/2022

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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