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Alexa Fluor® 647 anti-SOX17 Recombinant Antibody (for IHC and ICC)

Pricing & Availability
Clone
O92B6.Rec (See other available formats)
Regulatory Status
RUO
Other Names
Transcription factor SOX17, Transcription factor SOX 17, Transcription factor SOX-17, VUR3, SRY-Box17, SRY-Related HMG-Box Transcription Factor 17
Isotype
Mouse IgG1, κ
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Product Citations
publications
A. O92B6Rec_A647_Sox17_IHC-P_092624
IHC staining with Alexa Fluor® 647 anti-SOX17 was performed on formalin-fixed paraffin-embedded human endometrium tissue following antigen retrieval using Tris-EDTA pH 9.0 buffer (Cat. No. 422703). The tissue sections were incubated with Alexa Fluor® 647 mouse IgG1, κ isotype control (Cat. No. 400155) (panel A) or with Alexa Fluor® 647 anti-SOX17 Recombinant (clone O92B6.Rec) (panels B and C). Nuclei were counterstained with DAPI (Cat. No. 422801) (overlay in panels A and C). Scale Bar: 50 µm
  • A. O92B6Rec_A647_Sox17_IHC-P_092624
    IHC staining with Alexa Fluor® 647 anti-SOX17 was performed on formalin-fixed paraffin-embedded human endometrium tissue following antigen retrieval using Tris-EDTA pH 9.0 buffer (Cat. No. 422703). The tissue sections were incubated with Alexa Fluor® 647 mouse IgG1, κ isotype control (Cat. No. 400155) (panel A) or with Alexa Fluor® 647 anti-SOX17 Recombinant (clone O92B6.Rec) (panels B and C). Nuclei were counterstained with DAPI (Cat. No. 422801) (overlay in panels A and C). Scale Bar: 50 µm
  • B. O92B6Rec_A647_Sox17_ICC_092624
    OVCAR-3 cells (positive cell line, top panels), or HeLa cells (negative cell line, bottom panels) were fixed and permeabilized with 4% PFA and ice-cold methanol, and then intracellularly stained with Alexa Fluor® 674 anti-SOX17 Recombinant (clone O92B6.Rec) (right panels) or Alexa Fluor® 647 mouse IgG1, κ isotype control (Cat. No. 400155) (left panels). The cells were counterstained with DAPI (Cat. No. 422801) (blue) and Alexa Fluor® 488 anti-Cytokeratin (Cat. No. 628608) (green). The images were captured in a Revvity/Perkin Elmer Operetta CLS™ High Content Analysis System with a 63X objective. Scale bar 50 µm
Compare all formats See Alexa Fluor® 647 spectral data
Cat # Size Price Save
628159 25 µg ¥41,800
628160 100 µg ¥95,700
Description

SOX17 is an important member of the SOX family of transcription factors, which are defined by possession of a homologous sequence called the HMG (high mobility group) box. SOX genes are primarily involved in developmental processes including germ layer formation, cell-type specification, and organogenesis. SOX17 activates endodermal target genes, and is required for the formation of endoderm and blood vessel endothelium. SOX17 also plays critical roles in the maintenance of fetal and neonatal hematopoietic stem cells. Additionally, SOX17 is a regulator of human primordial germ cell fate.

SOX17 also acts as a tumor suppressor through suppression of Wnt signaling, and a reduction of SOX17 expression is found in gastrointestinal and colon carcinomas. Additionally, mutations in SOX17 can induce stem-like characteristics in tumor cells by causing SOX17 to dimerize with OCT4 and induce pluripotency transcriptional programs.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Recombinant
Host Species
Mouse
Immunogen
Recombinant protein expressed in E. Coli corresponding to residues 177-414 of human SOX17
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-P - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 5.0 - 10.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

We recommend this antibody for use in immunocytochemistry (ICC) and immunohistochemistry on formalin-fixed paraffin-embedded tissues (IHC-P). This product has been optimized for ICC and IHC-P applications.

For use in intracellular staining flow cytometry (ICFC), we recommend Cat. No. 628157 and 628158. This product has been optimized for flow cytometry application.

Antigen Details

Structure
SOX17 is a 414 amino acid protein with a predicted molecular weight of 44.1 kD.
Distribution

Nucleus

Function
Transcription factor
Interaction
Interacts with CTNNB1, LEF1, TCF4
Biology Area
Cell Biology, Signal Transduction, Transcription Factors
Antigen References
  1. Wegner M. 1999. Nucleic Acids Res. 27:1409-20.
  2. Du YC, et al. 2009. Gastroenterology. 137:1346-57.
  3. Zorn AM, et al. 1999. Mol Cell. 4:487-98.
  4. Sinner D, et al. 2004. Development. 131:3069-80.
  5. Hudson C, et al. 1997. Cell. 91:397-405.
  6. Matsui T, et al. 2006. J Cell Sci. 119:3513-26.
  7. Kim I, et al. 2007. Cell. 130:470-83.
  8. Zhang W, et al. 2008. Cancer Res. 68:2764-72.
  9. Tan DS, et al. 2020. Semin Cancer Biol. 67:65-73.
  10. Irie N, et al. 2015. Cell. 160:253-68.
Gene ID
64321 View all products for this Gene ID
UniProt
View information about SOX17 on UniProt.org
Go To Top Version: 1    Revision Date: 09/26/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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