Alexa Fluor® 647 anti-TFEB Antibody, TFEB, W18197C

Clone: W18197C (See other available formats)
Regulatory Status: RUO
Other Names: Transcription Factor EB
Isotype: Rat IgG2a, κ
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Product Citations: publications

W18197C_Alexa-Fluor-647_-TFEB_Antibody_030923 — Daudi cells (positive control, filled histogram) and Jurkat cells (low expression control, open histogram) were fixed and permeabilized using True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) and intracellularly stained with Alexa Fluor® 647 anti-TFEB (clone W18197C), or Alexa Fluor® 647 rat IgG2a, κ isotype control (open histogram, dashed line) (representative histogram for both cell lines) (Cat No. 400526).

Compare all formats See Alexa Fluor® 647 spectral data

Cat #	Size	Price	Save
936105	25 tests	¥43,560
936106	100 tests	¥103,180

Description

The autophagy-lysosomal pathway is required for the quality control degradation of proteins and other macromolecules. The transcription factor EB (TFEB) plays a critical role in inducing expression of genes involved in autophagy and lysosomal biogenesis. The subcellular localization and activity of TFEB are regulated by mechanistic target of rapamycin (mTOR)-mediated phosphorylation. Under nutrient-replete conditions, mTORC1 phosphorylates TFEB promoting its cytosolic retention by 14-3-3 proteins and nuclear export through CRM1. Under nutrient deprivation, TFEB phosphorylation decreases, promoting its nuclear import and transactivation function. TFEB is frequently dysregulated in cancer, and is also involved in the clearance of intracellular pathogenic targets in a variety of diseases, such as Parkinson's and Alzheimer's, suggesting that novel therapeutic strategies could be based on the modulation of TFEB activity.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Partial recombinant human TFEB protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser: Red Laser (633 nm)
Application Notes: When TFEB is phosphorylated, a molecular weight increase may be observed by western blot (Martina JA, et al. 2013. J Cell Biol. 200:475).

Differences in the observed molecular weight across cell lines and tissues may be attributed to differences in basal TFEB phosphorylation in these samples.
RRID: AB_2936767 (BioLegend Cat. No. 936105)
AB_2936767 (BioLegend Cat. No. 936106)

Antigen Details

Structure: TFEB is a 476 amino acid protein with a predicted molecular weight of 53 kD.
Distribution: Nucleo-cytoplasmic/Ubiquitously expressed
Function: Transcription Factor required for inducing expression of genes involved in autophagy and lysosomal biogenesis.
Biology Area: Cell Biology, Transcription Factors
Antigen References: Napolitano G, et al. 2018. Nat Commun. 9:3312.

Peña-Llopis S, et al. 2011. EMBO J. 30:3242.

David R, et al. 2011. Nat Rev Mol Cell Biol. 12:404.

Settembre C, et al. 2011. Science. 332:1429.
Gene ID: 7942 View all products for this Gene ID
UniProt: View information about TFEB on UniProt.org

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Related Protocols

Intracellular Flow Cytometry Staining Protocol

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Other Formats

View All TFEB Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-TFEB	W18197C	WB,IP,ICFC,IHC-P
PE anti-TFEB	W18197C	ICFC
Alexa Fluor® 647 anti-TFEB	W18197C	ICFC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 03/09/2023

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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