Loading...

Alexa Fluor® 647 Streptavidin

Pricing & Availability
Regulatory Status
RUO
Other Names
Streptavidin-Alexa Fluor® 647, Streptavidin-AF647, SAV
Ave. Rating
Submit a Review
Product Citations
publications
Strepavidin_A647_Antibody_FC_013114
Human peripheral blood lymphocytes were stained with biotinylated CD3 (filled histogram) or biotinylated mouse IgG1 isotype control (open histogram), followed by SAV-Alexa Fluor® 647.
  • Strepavidin_A647_Antibody_FC_013114
    Human peripheral blood lymphocytes were stained with biotinylated CD3 (filled histogram) or biotinylated mouse IgG1 isotype control (open histogram), followed by SAV-Alexa Fluor® 647.
See Alexa Fluor® 647 spectral data
Cat # Size Price Save
405237 100 µg ¥45,320
Description

Streptavidin binds to biotin with high affinity. Streptavidin-Alexa Fluor® 647 is useful for detecting biotinylated antibodies. The excitation of Alexa Fluor® 647 by 633 nm / 635 nm laser light induces a light emission maximum of 668 nm.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse, Rat, All Species
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
Streptavidin was conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml (concentration relates to the Streptavidin only component of the conjugate)
Storage & Handling
The streptavidin-Alexa Fluor® 647 solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

ICFC - Verified
Recommended Usage

This streptavidin product is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the Alexa Fluor® 647 conjugation. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Application Notes

Streptavidin-Alexa Fluor® 647 is useful as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. The average molecular weight of Streptavidin-Alexa Fluor® 647 is 60 kD and Streptavidin alone is 52 kD.

Application References

(PubMed link indicates BioLegend citation)
  1. Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245.
  2. Nishimoto KP,et al. 2008. J. Immunol. 181:4010.PubMed
  3. Niki T, et al. 2009. J. Biol. Chem. 284:32344. PubMed
  4. Shibui A, et al. 2011. Exp Parasitol. 129:318. PubMed
  5. Scatizzi JC, et al. 2012. J. Immunol. 188:3307. PubMed
  6. Yamakawa N, et al. 2012. Int Immunol. PubMed
  7. Shibata T, et al. 2012. Int Immunol. 24:613. PubMed
  8. Baccala R, et al. 2012. J. Immunol. 189:5976. PubMed
  9. Grevers LC, et al. 2013. Ann Reheum Dis. 72:278. PubMed
  10. Woo SJ, et al. 2013. J Leukoc Biol. 93:363. PubMed
  11. Ashbaugh JJ, et al. 2013. J. Immunol. 190:4525. PubMed
  12. Reading JL, 2013. J. immunol. 190:4542. Pubmed.
  13. Kanno A, et al. 2013. Int Immunol. 25:413. PubMed
  14. Gunaydin G, et al. 2014. Vaccine. 32:470. PubMed
  15. Shade KT, et al. 2015. J Exp Med. 212:457. PubMed
Product Citations
  1. Janeczek A, et al. 2017. Nanomedicine. 10.2217/nnm-2016-0386. PubMed
  2. Hu Q, et al. 2022. Cell Rep. 40:111035. PubMed
  3. Weskamm LM, et al. 2022. Cell Rep Med. 3:100685. PubMed
  4. Wang Z, et al. 2022. J Exp Med. 219: . PubMed
  5. Taft JM, et al. 2022. Cell. 185:4008. PubMed
  6. Schaefer-Babajew D, et al. 2023. Nature. 613:735. PubMed
  7. Voss LF, et al. 2023. Front Immunol. 13:1021370. PubMed
  8. Weskamm LM, et al. 2022. STAR Protoc. 3:101902. PubMed
  9. Bettini E, et al. 2022. STAR Protoc. 3:101840. PubMed
  10. Shilts J, et al. 2023. PLoS Biol. 21:e3001959. PubMed
  11. Bednarski E, et al. 2022. MBio. 13:e0084022. PubMed
  12. Tas JMJ, et al. 2022. Immunity. 55:1856. PubMed
  13. Deisher A, et al. 2022. STAR Protoc. 3:101663. PubMed
  14. Melzi E, et al. 2022. Immunity. 55:2168. PubMed
  15. Shibata K, et al. 2022. Nat Commun. 13:6948. PubMed
  16. Agudelo M, et al. 2021. J Exp Med. :218. PubMed
  17. Gaebler C, et al. 2021. Nature. 639:591. PubMed
  18. Pobezinskaya EL et al. 2019. Front Immunol. 1.079861111 . PubMed
  19. Schiesser JV, et al. 2021. Sci Rep. 11:8315. PubMed
  20. Agudelo M, et al. 2022. J Exp Med. 219:. PubMed
  21. Noreng S, et al. 2022. Nat Commun. 13:6079. PubMed
  22. Needham BD, et al. 2022. Nature. 602:647. PubMed
  23. Shade K, et al. 2015. J Exp Med. 212:457. PubMed
  24. Biram A, et al. 2020. Cell Rep. 30:1910. PubMed
  25. Sokol CL et al. 2018. Immunity. 49(3):449-463 . PubMed
  26. Zhou D, et al. 2022. Nat Commun. 13:5174. PubMed
  27. Zhang R, et al. 2021. MAbs. 13:1904546. PubMed
  28. Wang Z, et al. 2021. bioRxiv. . PubMed
  29. Glaros V, et al. 2021. Immunity. 54:2005. PubMed
  30. Yap K, et al. 2022. STAR Protoc. 3:101139. PubMed
  31. Cho A, et al. 2021. Nature. Online ahead of print. PubMed
  32. Charles TP, et al. 2021. PLoS Pathog. 17:e1009257. PubMed
  33. Van Den Berge N, et al. 2019. Acta Neuropathol. 138(4):535. PubMed
  34. Kacherovsky N, et al. 2019. Nat Biomed Eng. 0.66875. PubMed
  35. Alameh MG, et al. 2021. Immunity. 54:2877. PubMed
  36. Gilpin TE, et al. 2021. J Immunol. 207:1065. PubMed
  37. Van Simaeys D, et al. 2022. Nat Commun. 13:1815. PubMed
  38. Biram A, et al. 2020. Bio Protoc. e3562:10. PubMed
  39. Lee M, et al. 2020. Elife. :9. PubMed
  40. Duong BTV, et al. 2020. EBioMedicine. 61:103031. PubMed
  41. Wang X, et al. 2021. EMBO J. 40:e105926. PubMed
  42. Kulkarni N, et al. 2017. Eur J Appl Physiol.. 10.1007/s00421-017-3704-z. PubMed
  43. Wang Z, et al. 2021. Nature. 592:616. PubMed
  44. Puentes-Mestril C, et al. 2021. J Neurosci. 41:5386. PubMed
  45. Wang Z, et al. 2022. J Exp Med. 219:. PubMed
  46. Wang Z, et al. 2021. Nature. 595:426. PubMed
  47. Lederer K, et al. 2022. Cell. . PubMed
  48. Wang Z, et al. 2020. Elife. 9:00. PubMed
  49. Li D, et al. 2020. Immunohorizons. 0.661805556. PubMed
  50. Gaebler C, et al. 2020. bioRxiv. . PubMed
  51. Plewes MR, et al. 2020. FASEB J. 5299:34. PubMed
  52. Chmielewski M and Abken H 2017. Cell Rep.. 10.1016/j.celrep.2017.11.063. PubMed
  53. Cho A, et al. 2022. J Exp Med. 219:. PubMed
  54. Frohlich J, et al. 2020. Aging (Albany NY). 12:20024. PubMed
  55. Pabbisetty S, et al. 2016. Proc Natl Acad Sci U S A. 113: E4662 - E4670. PubMed
  56. Yoshikawa S, et al. 2016. Sci Rep. 6:18738. PubMed
  57. Tsai S, et al. 2018. Cell Metab. 28:922. PubMed
Go To Top Version: 5    Revision Date: 06/18/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account