Alexa Fluor® 660 anti-human CD19 Antibody, CD19, HIB19

Clone: HIB19 (See other available formats)
Regulatory Status: RUO
Workshop: V CD19.11
Other Names: B4
Isotype: Mouse IgG1, κ
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Product Citations: publications

HIB19_AF660_CD19_Antibody_1_011024 — Human peripheral blood lymphocytes were stained with anti-human CD20 APC/Cyanine7 and anti-human CD19 (clone HIB19) Alexa Fluor® 660 (left), or stained with anti-human CD20 APC/Cyanine7 only (right).

Compare all formats See Alexa Fluor® 660 spectral data

Cat #	Size	Price	Save
302291	25 tests	¥28,600
302292	100 tests	¥78,100

Description

CD19 is a 95 kD type I transmembrane glycoprotein also known as B4. It is a member of the immunoglobulin superfamily expressed on B-cells (from pro-B to blastoid B cells, absent on plasma cells) and follicular dendritic cells. CD19 is involved in B cell development, activation, and differentiation. CD19 forms a complex with CD21 (CR2) and CD81 (TAPA-1), and functions as a BCR co-receptor.

Product Details

Technical data sheet

Product Details

Verified Reactivity

Human

Reported Reactivity

Chimpanzee

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 660 under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 660 has an excitation maximum of 663 nm, and a maximum emission of 690 nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser

Red Laser (633 nm)

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections⁸ and blocking of B cell proliferation. Clone HIB19 is not recommended for formalin-fixed paraffin-embedded sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 302267 & 302268).

Clone HIB19 partially blocks anti-human CD19 clones 4G7 and SJ25C1 staining based on in-house testing

Application References (PubMed link indicates BioLegend citation)

Schlossman S, et al. 1995. Leucocyte Typing V. Oxford University Press. New York.
Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
Bradbury L, et al. 1993. J. Immunol. 151:2915.
Joseph A, et al. 2010. J. Virol. 84:6645. PubMed
Wang X, et al. 2010. Haematologica. 95:884. (FC) PubMed
Walker JD, et al. 2009. J. Immunol. 182:1548. (Block) PubMed
Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
Hansen A, et al. 2002. Arthritis Rheum. 46:2160. (IHC)
Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)

RRID

AB_3106013 (BioLegend Cat. No. 302291)
AB_3106013 (BioLegend Cat. No. 302292)

Antigen Details

Structure: Ig superfamily, type I transmembrane glycoprotein, 95 kD
Distribution: B lineage (except plasma cells), follicular dendritic cells
Function: B cell activation and differentiation
Ligand/Receptor: Forms complex with CD21 (CR2) and CD81 (TAPA-1), BCR coreceptor
Cell Type: B cells, Dendritic cells
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Tedder T, et al. 1994. Immunol. Today 15:437.
2. Bradbury L, et al. 1993. J. Immunol. 151:2915.
Gene ID: 930 View all products for this Gene ID
UniProt: View information about CD19 on UniProt.org

Documentation

Reviews

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Description	Clone	Applications

Related Pages & Pathways

Pathways

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD19 Reagents Request Custom Conjugation

Description	Clone	Applications
APC anti-human CD19	HIB19	FC
Biotin anti-human CD19	HIB19	FC
FITC anti-human CD19	HIB19	FC
PE anti-human CD19	HIB19	FC
PE/Cyanine5 anti-human CD19	HIB19	FC
Purified anti-human CD19	HIB19	FC,CyTOF®,IHC-F
APC/Cyanine7 anti-human CD19	HIB19	FC
PE/Cyanine7 anti-human CD19	HIB19	FC
Alexa Fluor® 488 anti-human CD19	HIB19	FC,ICC
Alexa Fluor® 647 anti-human CD19	HIB19	FC,IHC-F
Pacific Blue™ anti-human CD19	HIB19	FC
Alexa Fluor® 700 anti-human CD19	HIB19	FC
PerCP anti-human CD19	HIB19	FC
PerCP/Cyanine5.5 anti-human CD19	HIB19	FC
Brilliant Violet 421™ anti-human CD19	HIB19	FC,ICC,IHC-F
Brilliant Violet 570™ anti-human CD19	HIB19	FC
Brilliant Violet 650™ anti-human CD19	HIB19	FC
Brilliant Violet 785™ anti-human CD19	HIB19	FC
Brilliant Violet 510™ anti-human CD19	HIB19	FC
Brilliant Violet 605™ anti-human CD19	HIB19	FC
Brilliant Violet 711™ anti-human CD19	HIB19	FC
Purified anti-human CD19 (Maxpar® Ready)	HIB19	FC,CyTOF®
Alexa Fluor® 594 anti-human CD19	HIB19	ICC,IHC-F
PE/Dazzle™ 594 anti-human CD19	HIB19	FC
PE anti-human CD19	HIB19	FC
APC/Fire™ 750 anti-human CD19	HIB19	FC
Pacific Blue™ anti-human CD19	HIB19	FC
APC anti-human CD19	HIB19	FC
PE/Cyanine7 anti-human CD19	HIB19	FC
TotalSeq™-A0050 anti-human CD19	HIB19	PG
Brilliant Violet 750™ anti-human CD19	HIB19	FC
TotalSeq™-B0050 anti-human CD19	HIB19	PG
TotalSeq™-C0050 anti-human CD19	HIB19	PG
PerCP/Cyanine5.5 anti-human CD19	HIB19	FC
Spark NIR™ 685 anti-human CD19	HIB19	FC
Ultra-LEAF™ Purified anti-human CD19	HIB19	FC,CyTOF®,Block,IHC
APC/Fire™ 810 anti-human CD19	HIB19	FC
PE/Fire™ 640 anti-human CD19	HIB19	FC
PE/Fire™ 700 anti-human CD19	HIB19	FC
TotalSeq™-D0050 anti-human CD19	HIB19	PG
Spark YG™ 593 anti-human CD19	HIB19	FC
GMP Pacific Blue™ anti-human CD19	HIB19	FC
Spark Violet™ 423 anti-human CD19	HIB19	FC
GMP PE anti-human CD19	HIB19	FC
GMP APC anti-human CD19	HIB19	FC
KIRAVIA Blue 520™ anti-human CD19	HIB19	FC
GMP PerCP/Cyanine5.5 anti-human CD19	HIB19	FC
GMP PE/Cyanine7 anti-human CD19	HIB19	FC
Spark Violet™ 500 anti-human CD19	HIB19	FC
PE/Fire™ 810 anti-human CD19	HIB19	FC
Spark Violet™ 423 anti-human CD19	HIB19	FC
Spark UV™ 387 anti-human CD19	HIB19	FC
APC/Fire™ 750 anti-human CD19	HIB19	FC
PE/Cyanine5 anti-human CD19	HIB19	FC
Alexa Fluor® 660 anti-human CD19	HIB19	FC
PerCP/Fire™ 806 anti-human CD19	HIB19	FC
PerCP/Fire™ 780 anti-human CD19	HIB19	FC
Spark PLUS UV395™ anti-human CD19	HIB19	FC
Spark Red™ 718 anti-human CD19	HIB19	FC
FITC anti-human CD19	HIB19	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 01/10/2024

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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