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Alexa Fluor® 700 anti-human HLA-A2 Antibody

Pricing & Availability
Clone
BB7.2 (See other available formats)
Regulatory Status
RUO
Other Names
MHC-class I
Isotype
Mouse IgG2b, κ
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Product Citations
publications
BB7.2_AF700_HLA-A2_Antibody_FC_1_011713
Peripheral blood lymphocytes from HLA-A2 positive (top) and HLA-A2 negative (bottom) donors were stained with anti-human HLA-A2 (clone BB7.2) Alexa Fluor® 700 (filled histograms) or mouse IgG2b Alexa Fluor® 700 isotype control (open histograms).
  • BB7.2_AF700_HLA-A2_Antibody_FC_1_011713
    Peripheral blood lymphocytes from HLA-A2 positive (top) and HLA-A2 negative (bottom) donors were stained with anti-human HLA-A2 (clone BB7.2) Alexa Fluor® 700 (filled histograms) or mouse IgG2b Alexa Fluor® 700 isotype control (open histograms).
  • BB7.2_AF700_HLA-A2_Antibody_FC_2_011713
Compare all formats See Alexa Fluor® 700 spectral data
Cat # Size Price Save
343317 25 tests ¥28,380
343318 100 tests ¥73,700
Description

HLA-A2 is most common in Northern Asia and North America populations. MHC class I antigens associated with β2-microglobulin are expressed by all human nucleated cells. MHC class I molecules are involved in presentation of antigens to CD8+ T cells, playing an important role in cell-mediated immune responses and tumor surveillance.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Papain solubilized HLA-A2
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

The BB7.2 antibody recognizes human leukocyte antigen (HLA) A2 which is a subset of MHC-class I molecules encoded by A*02 alleles.

Additional reported applications (for the relevant formats) include: immunoprecipitation3.

Application References

(PubMed link indicates BioLegend citation)
  1. Brodsky FM, et al. 1979. Immunol. Rev. 47:3.
  2. Parham P and Brodsky FM. et al. 1981. Hum. Immunol. 3:277.
  3. Lubben NB, et al. 2007. Mol Biol Cell. 18:3351. (IP)
RRID
AB_2561784 (BioLegend Cat. No. 343317)
AB_2561784 (BioLegend Cat. No. 343318)

Antigen Details

Structure
Ig superfamily
Distribution

A*02 individuals leukocytes

Function
Antigen presentation
Ligand/Receptor
CD8, CD3/TCR
Cell Type
Leukocytes
Biology Area
Immunology
Molecular Family
MHC Antigens
Gene ID
3105 View all products for this Gene ID
UniProt
View information about HLA-A2 on UniProt.org
Go To Top Version: 3    Revision Date: 06/27/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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