Anti-CD230 Prion Antibody anti-CD230 (Prion)

Clone: 3F4 (See other available formats)
Regulatory Status: RUO
Other Names: Major prion protein, p27-30, CD230 antigen, prion protein PrP, prion-related protein
Previously: Signet Catalog# 9600-02
Signet Catalog# 9600-05
Signet Catalog# 9600-10
Covance Catalog# SIG-39600
Isotype: Mouse IgG2a, κ
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Product Citations: publications

3F4_Ascites_CD230_Antibody_101718 — Human peripheral blood lymphocytes were stained with anti-CD230 (Prion) antibody (clone 3F4, filled histogram) or Alexa Fluor® 647 Mouse IgG2a, κ isotype control antibody (Cat. No. 400234) (open histogram). The anti-CD230 (Prion) antibody (clone 3F4) staining was followed by staining with Alexa Fluor® 647 conjugated goat anti-mouse IgG(Cat. No. 405322).

Cat #	Size	Price	Save
800307	200 µL	¥231,880

Description

Prions cause neurodegenerative disease by aggregating extracellularly within the central nervous system which disrupt the normal tissue structure. This disruption is characterized by "holes" in the tissue with resultant spongy architecture. Two conformational isoforms exist, the normal cellular isoform (PrPC) and the infectious, scrapie isoform (PrPSC). Other histological changes include astrogliosis and the absence of an inflammatory reaction. Neurodegenerative symptoms can include convulsions, dementia, ataxia (balance and coordination dysfunction), and behavioral or personality changes.

All known prion diseases are collectively called transmissible spongiform encephalopathies (TSEs). Prion (PrP) is highly conserved through mammals and comparison between primates ranges from 92.9-99.6% similarity in amino acid sequence. The human protein structure consists of a globular domain with three α-helices and a two-strand antiparallel β-sheet, an NH2-terminal tail, and a short COOH-terminal tail. A glycosylphosphatidylinositol (GPI) membrane anchor at the COOH-terminal tethers PrP to cell membranes. This anchor is integral to the transmission of conformational change; secreted PrP lacking the anchor component is unaffected by the infectious isoform. PrPSC accumulates in compact, protease-resistant aggregates within neural tissue and has a different secondary and tertiary structure from PrPC, but an identical primary sequence.

The primary sequence of PrP is 253 amino acids long before posttranslational modification. Signal sequences in the amino- and carboxy- terminal ends are removed posttranslationally, resulting in a mature length of 208. For human and Syrian hamster PrP, two glycosylated sites exist on helices 2 and 3 at Asn181 and Asn197. Murine PrP has glycosylation sites as Asn180 and Asn196. A disulfide bond exists between Cys179 of the second helix and Cys214 of the third helix (human PrPC numbering).

The precise function of PrP is not yet known, but it is possibly involved in the transport of ionic copper to cells from the surrounding environment. Researchers have also proposed roles for PrP in cell signaling or in the formation of synapses.

Spatial learning, a predominantly hippocampal-function, is decreased in PrP null mice and can be recovered with the reinstatement of PrP in neurons; indicating that loss of PrP function is the cause. PrP is present in both pre- and post-synaptic neuron cells, and the greatest concentration is in the pre-synaptic cells. Some research indicates PrP involvement in neuronal development, differentiation, and neurite outgrowth. The PrP-activated signal transduction pathway is associated with axon and dendritic outgrowth with a series of kinases.

Though most attention is focused on PrP’s presence in the nervous system, it is also abundant in immune system tissue. PrP immune cells include haematopoietic stem cells, mature lymphoid and myeloid compartments, and certain lymphocytes; also, it has been detected in natural killer cells, platelets, and monocytes. T cell activation is accompanied by a strong up-regulation of PrP, though it is not requisite. The lack of immuno-response to transmissible spongiform encephalopathies (TSE), neurodegenerative diseases caused by prions, could stem from the tolerance for PrPSc.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Preparation: Ascites
Concentration: The concentration is not quantified as this product is sold as undiluted crude mouse ascites fluid. The concentration might vary from lot-to-lot and an estimated concentration would be 1-3 mg/ml.
Storage & Handling: Do not store antibody diluted below 50 µg/mL in the absence of protein (i.e.: add 2% bovine serum albumin). It is suggested that the total volume is aliquoted upon initial thaw. The antibody is best stored undiluted at -20°C to -70°C.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 1:1600 - 1:3200 dilution. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: 3F4 is reactive to amino acid residues 109-112 of prion protein (PrP) from humans, hamsters, and felines. It recognizes both protease sensitive and protease resistant forms of PrP (after denaturing).
RRID: AB_2565333 (BioLegend Cat. No. 800307)

Antigen Details

Biology Area: Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family: Prion (CD230)
Antigen References: 1. Asante EA, et al. 2015. Nature 522:478.
2. Mead S, et al. 2013. N. Engl. J. Med. 369:1904.
3. Chakrabarti O, Hegde RS. 2009. Cell 137:1136.
4. Wadsworth JD, et al. 2004. Science 306:1793.
Gene ID: 5621 View all products for this Gene ID
UniProt: View information about CD230 on UniProt.org

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Cell Surface Flow Cytometry Staining Protocol

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Other Formats

View All CD230 (Prion) Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-CD230 (Prion)	3F4	FC,ELISA,WB,IHC-F,IP
Anti-CD230 (Prion)	3F4	FC
Biotin anti-CD230 (Prion)	3F4	FC
FITC anti-human CD230 (Prion)	3F4	FC
APC anti-human CD230 (Prion)	3F4	FC
PE anti-human CD230 (Prion)	3F4	FC

Certificate of Analysis

Go To Top Version: 4 Revision Date: 10/17/2018

For Research Use Only. Not for diagnostic or therapeutic use.

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