- Clone
- 8RB13 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- RNAP subunit beta, rpoB, groN, nitB, rif, ron, stl, stv, tabD, RNA polymerase subunit beta, Transcriptase subunit beta
- Previously
-
NeoClone Catalog# S0004
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
RNA polymerase (RNAP or RNApol) is an enzyme that produces primary transcript RNA. The β subunit is the second-largest subunit, and is encoded by the roB gene. RNA polymerase β is a subunit which, along wtih α (rpoC), limits core polymerase expression.
Product DetailsProduct Details
- Verified Reactivity
- E. coli
- Reported Reactivity
- Other species
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Purified recombinant E. coli RNA polymerase β subunit.
- Formulation
- 50% anti- E. coli RNA Polymerase antibody conjugated resin is supplied in 1X PBS and 0.09% NaN3. The volume specified for each catalog number indicates the volume of resin included.
- Storage & Handling
- Store at 4°C until use. Use at room temperature.
- Application
-
IP - Quality tested
- Recommended Usage
-
Resin binding capacity is approximately 0.15 mg/mL. To elute, use TE, 0.7M ammonium sulfate, 40% propylene glycol.
- Application Notes
-
Clone 8RB13 is extremely useful for detecting or purifying core RNAP from a broad range of bacteria. The purified polymerase is highly active for in vitro transcription.
Additional reported applications (for the relevant formats) include: immunoprecipitation3. The epitope of clone 8RB13 has been mapped to the sequence PEEKLLRAIFGEKAS3. -
Application References
(PubMed link indicates BioLegend citation) -
- Bergendahl V, et al. 2003. Protein Expr. Purif. 31:155.
- Burgess RR and Thompson NE. 2002. Curr. Opin. Biotechnol. 13:304.
- Stalder, ES. et al. 2011. Protein Expr. Purif. 77(1):26-33. (Epitope, ELISA, IP, WB)
- RRID
-
AB_3105970 (BioLegend Cat. No. 673801)
Antigen Details
- Structure
- 1252 amino acids with a predicted molecular weight of approximately 150 kD.
- Distribution
-
Bacteria.
- Function
- Transcriptional regulator in bacteria.
- Biology Area
- Cell Biology, Transcription Factors
- Molecular Family
- Nuclear Markers
- Antigen References
-
1. Thompson NE, et al. 1992. Biochemistry 31:7003.
2. Jerald Hurwitz, et al. 2005. J. Biol. Chem. 280:42477.
3. Hirata A, et al. 2008. Nature. 451:851.
4. Bergendahl IV, et al. 2003. Protein Expr. Purif. 31:155.
5. Burgess RR and Thompson NE. 2002. Curr. Opin. Biotechnol. 13:304. - Gene ID
- 948488 View all products for this Gene ID
- UniProt
- View information about RNA Polymerase beta on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All RNA Polymerase β Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Anti-E. coli RNA Polymerase ImmunoAffinity Resin | 8RB13 | IP |
Anti-E. coli RNA Polymerase β | 8RB13 | WB,ELISA,IP |
Purified anti-E. coli RNA Polymerase β | 8RB13 | WB,ELISA,IP |
Direct-Blot™ HRP anti-E. coli RNA Polymerase β | 8RB13 | WB |
Compare Data Across All Formats
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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