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- Clone
- UCHT1; RPA-T4; RPA-T8;
- Regulatory Status
- RUO
- Isotype
- Mouse IgG1 (all clones)
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Human peripheral blood lymphocytes were stained with anti-human CD3 PE/Cyanine5, CD4 PE, CD8 FITC cocktail.
CD3: UCHT1 antibody reacts with a combinatorial epitope of CD3ε, a 20 kD chain of the CD3/T-cell receptor (TCR) complex found on all mature T lymphocytes, NK-T cells and some thymocytes.
CD4: RPA-T4 antibody reacts with CD4, a 55 kD single-chain type I transmembrane glycoprotein expressed on most thymocytes, a subset of T cells, and monocytes/macrophages.
CD8: RPA-T8 antibody reacts with CD8a, a 32-34 kD type I glycoprotein. CD8a forms a heterodimer or homodimer with CD8b. CD8 also known as T8 and Leu2, is a member of the immunoglobulin superfamily found on the majority of thymocytes, a subset of peripheral blood T cells, and NK cells (express almost exclusively CD8a homodimers).
Product Details
- Verified Reactivity
- Human
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
- Preparation
- This reagent is a combination of PE/Cyanine5 conjugated UCHT1, PE conjugated RPA-T4 and FITC conjugated RPA-T8 at optimal concentration for three-color flow cytometric analysis.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
-
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. To obtain lot-specific expiration date, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
This product has a shelf-life of 12 months or less. Please contact our technical support team for lot specific CoA and expiration date inquiries of this product. - Application
-
FC - Quality tested
- Recommended Usage
-
For flow cytometric staining, the suggested use of this reagent is 20 µl per million cells or 20 µl per 100 µl of whole blood.
- Application Notes
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis.
Note:
Single color controls are required to perform instrument compensation and are not included in the kit. - Product Citations
-
- RRID
-
AB_316003 (BioLegend Cat. No. 319001)
Antigen Details
- Distribution
-
T-cell receptor (TCR) complex found on all mature T lymphocytes, NK-T cells and some thymocytes
- Cell Type
- NKT cells, T cells, Thymocytes, Tregs
- Biology Area
- Immunology
- Antigen References
-
1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.
3. Lanier L, et al. 1986. J. Immunol. 137:2501-2507.
4. Center D, et al. 1996. Immunol. Today 17:476.
5. Gaubin M, et al. 1996. Eur. J. Clin. Chem. Clin. Biochem. 34:723. - Gene ID
- 920 View all products for this Gene ID 916 View all products for this Gene ID 925 View all products for this Gene ID
Related FAQs
Other Formats
View All Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Anti-human CD3 PE-Cyn5/CD4 PE/CD8 FITC Cocktail | UCHT1; RPA-T4; RPA-T8 | FC |
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Anti-human CD3 PE-Cyn5/CD4 PE/CD8 FITC Cocktail
Human peripheral blood lymphocytes were stained with anti-hu...
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