Anti-Synaptobrevin VAMP Antibody anti-Synaptobrevin (VAMP)

Clone: SP10 (See other available formats)
Regulatory Status: RUO
Other Names: Vesicle-associated membrane protein 1, synaptobrevin-1
Previously: Covance Catalog# MMS-616R
Isotype: Mouse IgG2b, κ
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Product Citations: publications

Western blot of anti-Synaptobrevin (VAMP) antibody (clone SP10). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with a 1:2000 dilution of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgM. Enhanced chemiluminescence was used as the detection system.

Cat #	Size	Price	Save
837001	500 µL	¥96,140

Description

Synaptobrevins, also known as VAMPs, along with syntaxins and the synaptosomal-associated protein SNAP25, form the protein complex that is involved with the docking and fusion of synaptic vesicles with the presynaptic membrane. Synaptobrevins have a molecular weight of 18 kD, and are also involved in the formation of the SNARE complexes. The synaptobrevins are degraded by the protein tetanospasmin, from the tetanus-causing bacterium Clostridium tetani, as well as botulinum toxin from Clostridium botulinum.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: This antibody was raised against a crude synaptic preparation of post mortem human brain.
Preparation: Ascites
Concentration: The concentration is not quantified as this product is sold as undiluted crude mouse ascites fluid. The concentration might vary from lot-to-lot and an estimated concentration would be 1-3 mg/ml.
Storage & Handling: Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. For long-term storage, keep the antibody at -80°C.
Application: WB - Quality tested
IHC-P, ELISA, ICC - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution of this reagent is 1:1000 - 1:2000. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: SP10 reacts with native, denatured and recombinant synaptic vesicle proteins synaptobrevin/VAMP-1 (aa 1-118) and VAMP-2 expressed in a bacterial system (aa 33-96). The antibody is particularly valuable for immunocytochemical studies of the brain due to its stability in formalin sections.
RRID: AB_2565369 (BioLegend Cat. No. 837001)

Antigen Details

Structure: Synaptobrevin 1 (VAMP1) is a 118 amino acid protein with an apparent molecular mass of 13kD. Synaptobrevin 2 (VAMP2) is a 116 amino acid protein with an apparent molecular mass of 12.5 kD.
Biology Area: Cell Biology, Neuroscience, Synaptic Biology
Molecular Family: Presynaptic proteins
Gene ID: 6843 View all products for this Gene ID
UniProt: View information about Synaptobrevin on UniProt.org

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Western Blotting Protocol

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Other Formats

View All Synaptobrevin (VAMP) Reagents Request Custom Conjugation

Description	Clone	Applications
Anti-Synaptobrevin (VAMP)	SP10	WB,IHC-P,ELISA,ICC
Purified anti-Synaptobrevin (VAMP)	SP10	WB,IHC-P,ELISA,ICC

Certificate of Analysis

Go To Top Version: 4 Revision Date: 10/29/2021

For Research Use Only. Not for diagnostic or therapeutic use.

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