APC anti-human Arginase I Antibody, Arginase I, 14D2C43

Clone: 14D2C43 (See other available formats)
Regulatory Status: RUO
Other Names: ARG1, Liver Arginase, Type 1 Arginase, Arginase-1, Liver-Type Arginase
Isotype: Mouse IgG2b, κ
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Product Citations: publications

Human peripheral blood was surface stained with CD15 Pacific Blue™ and CD16 FITC, fixed, permeabilized, and then intracellularly stained with Arginase I (clone 14D2C43) APC (filled histogram) or mouse IgG2b, κ APC isotype control (open histogram). Histogram was gated on the neutrophil (CD15⁺CD16⁺) population.

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Cat #	Size	Price	Save
369705	25 tests	¥31,240
369706	100 tests	¥75,460

Description

Arginase I, also known as ARG1, is a 34.7kD protein expressed by neutrophils and myeloid-derived suppressor cells (MDSCs). There are two isoforms that are differentiated based on their tissue distribution and subcellular localization. Arginase I converts L-arginine into L-ornithine and urea; it is the final enzyme in the urea cycle. While mostly found in the liver, it can also be expressed in cells lacking a comprehensive urea cycle. Also, it contributes to vasodilation and vascular function. Arginase I is also reported to be involved in MDSC mediated suppression of T cell proliferation. Arginase I has been implicated in hyperargininemia (decreased function of arginase I) and Q fever.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Full length recombinant protein expressed in E.coli.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Red Laser (633 nm)
RRID: AB_2814328 (BioLegend Cat. No. 369705)
AB_2814328 (BioLegend Cat. No. 369706)

Antigen Details

Structure: Belongs to the ureohydrolase family and has a molecular mass of 34.7 kD.
Distribution: Neutrophils and myeloid-derived suppressor cells (MDSC).
Function: Converts L-arginine into L-ornithine and urea.
Interaction: Arginine.
Cell Type: Neutrophils
Biology Area: Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family: Enzymes and Regulators
Antigen References: 1. Munder M, et al. 2005. Blood 105:2549.
2. Luckner-Minden C, et al. 2010. JLB. 87:1125.
3. Holowatz L, et al. 2006. Journal of Physiology 574:573.
4. Sin YY, et al. 2013. PLoS One 8:11.
5. Benoit M, et al. 2008. Eur. J. Immunol. 4:1065.
Gene ID: 383 View all products for this Gene ID
UniProt: View information about Arginase I on UniProt.org

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Intracellular Flow Cytometry Staining Protocol

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Other Formats

View All Arginase I Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human Arginase I	14D2C43	ICFC
PE anti-human Arginase I	14D2C43	ICFC
APC anti-human Arginase I	14D2C43	ICFC
PE/Cyanine7 anti-human Arginase I	14D2C43	ICFC
PerCP/Cyanine5.5 anti-human Arginase 1	14D2C43	ICFC

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 08/15/2019

For Research Use Only. Not for diagnostic or therapeutic use.

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