APC anti-human CD117 (c-kit) Antibody, CD117, S18022G

Clone: S18022G (See other available formats)
Regulatory Status: RUO
Other Names: Stem cell factor receptor, c-kit, mast cell growth factor receptor, steel factor receptor
Isotype: Mouse IgG2a, κ
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S18022G_APC_CD117_Antibody_100920 — Kasumi-3 cells (human acute myeloblastic leukemia cell line) were stained with anti-human CD117 (c-kit) (clone S18022G) APC (filled histogram) or mouse IgG2a, κ APC isotype control (open histogram).

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Cat #	Size	Price	Save
375203	25 tests	¥31,240
375204	100 tests	¥67,760

Description

CD117 is a 145 kD protein tyrosine kinase also known as c-kit. It is a receptor for stem cell factor or c-kit ligand. CD117 is expressed on pluripotent hematopoietic progenitor cells (approximately 1-4% bone marrow cells), mast cells, and acute myeloid leukemia cells (AML). CD117 binding of c-kit ligand induces phosphorylation of CD117 and stimulates proliferation and survival of primitive hematopoietic stem cells as well as erythroid-committed and granulo-monocytic committed cells.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human CD117 transfectant
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)
Application Notes: Clone S18022G antibody can completely block the binding of clone 104D2 to the target, but it does not block the binding of clone w18195C to the target. Clone S18022G antibody binding to the target is not affected after cells are fixed by 4% PFA.
RRID: AB_2890808 (BioLegend Cat. No. 375203)
AB_2890808 (BioLegend Cat. No. 375204)

Antigen Details

Structure: Growth factor receptor with tyrosine kinase activity, subclass III, approximately 145 kD
Distribution: Pluripotent hematopoietic progenitor cells (approximately 1-4% bone marrow cells), mast cells, acute myeloid leukemic cells (AML)
Function: Receptor for stem cell factor. Induces proliferation and survival of primitive hematopoietic progenitors. Potent inducer of proliferation in erythroid-committed progenitor cells. Defects in CD117 have been linked to severe anemia and a decreased number of hematopoietic progenitor cells.
Ligand/Receptor: Stem cell factor (c-kit ligand )
Antigen References: 1. Giebel LB, et al. 1992. Oncogene 7:2207.
2. Furitsu T, et al. 1993. J. Clin. Invest. 92:1736.
Gene ID: 3815 View all products for this Gene ID
UniProt: View information about CD117 on UniProt.org

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Other Formats

View All CD117 Reagents Request Custom Conjugation

Description	Clone	Applications
Pacific Blue™ anti-human CD117 (c-kit) Antibody	S18022G	FC
PE/Cyanine7 anti-human CD117 (c-kit) Antibody	S18022G	FC
APC anti-human CD117 (c-kit)	S18022G	FC
Purified anti-human CD117 (c-kit) Antibody	S18022G	FC
PE anti-human CD117 (c-kit) Antibody	S18022G	FC

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 10/09/2020

For Research Use Only. Not for diagnostic or therapeutic use.

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