APC anti-human CD146 MUC18, Mel-CAM Antibody anti-CD146

Clone: SHM-57 (See other available formats)
Regulatory Status: RUO
Other Names: Muc-18, MCAM, Mel-CAM, S-endo
Isotype: Mouse IgG2a, κ
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SHM-57_APC_011812 — Human cervical cancer cell line, Hela, stained with CD146 (clone SHM-57) APC (filled histogram) or mouse IgG2a, κ APC isotype control (open histogram).

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Cat #	Size	Price	Save
342011	25 tests	¥32,780
342012	100 tests	¥69,080

Description

CD146 is a 118 kD integral transmembrane glycoprotein also known as MUC18, S-Endo, MCAM, and Mel-CAM (melanoma cell adhesion molecule). It belongs to the immunoglobulin superfamily. CD146 is expressed on melanoma cells, epithelial cells, endothelial cells, fibroblasts, activated T cells, multipotent mesenchymal stromal cells, and activated keratinocytes. CD146 mediates heterophilic cell adhesion and regulates monocyte transendothelial migration. The ligand of CD146 remains to be identified.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Red Laser (633 nm)
RRID: AB_10707636 (BioLegend Cat. No. 342011)
AB_10707636 (BioLegend Cat. No. 342012)

Antigen Details

Structure: 118 kD integral glycoprotein, Ig superfamily
Distribution: Melanoma cells, epithelial cells, endothelial cells, activated T cells, multipotent mesenchymal stromal cells
Function: Adhesion, monocytes transendothelial migration
Cell Type: Endothelial cells, Epithelial cells, Mesenchymal cells, Mesenchymal Stem Cells, T cells
Biology Area: Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Pickl WF, et al. 1997. J. Immunol. 158:2107
2. Weninger W, et al. 2000. J. Invest. Dermatol. 115:219
3. Sorrentino A, et al. 2008. Exp. Hematol. 36:1035
4. Bardin N, et al. 2009. Arterioscler. Thromb. Vasc. Biol. 29:746
Gene ID: 4162 View all products for this Gene ID
UniProt: View information about CD146 on UniProt.org

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Description	Clone	Applications

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Pathways

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Other Formats

View All CD146 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human CD146 (MUC18, Mel-CAM)	SHM-57	FC
PE anti-human CD146 (MUC18, Mel-CAM)	SHM-57	FC
Alexa Fluor® 488 anti-human CD146 (MUC18, Mel-CAM)	SHM-57	FC
PE/Cyanine7 anti-human CD146 (MUC18, Mel-CAM)	SHM-57	FC
APC anti-human CD146 (MUC18, Mel-CAM)	SHM-57	FC
PerCP/Cyanine5.5 anti-human CD146 (MUC18, Mel-CAM)	SHM-57	FC

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

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