APC anti-human CD326 (EpCAM) Recombinant Antibody, CD326, QA20A75

Clone: QA20A75 (See other available formats)
Regulatory Status: RUO
Other Names: Ep-CAM, tumor associated calcium signal transducer 1, epithelial cell surface antigen, epithelial glycoprotein 2, EGP2, adenocarcinoma associated antigen, TROP1
Isotype: Mouse IgG1, κ
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QA20A75_APC_CD326_Antibody_1_121323 — Human colon carcinoma cell line HT29 were stained with anti-human CD326 (EpCAM) recombinant (clone QA20A75) APC (filled histogram) or mouse IgG1, κ APC isotype control (open histogram).

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Cat #	Size	Price	Save
377503	25 tests	¥34,100
377504	100 tests	¥70,400

Description

CD326 is also known as Ep-CAM, tumor associated calcium signal transducer 1, epithelial cell surface antigen, epithelial glycoprotein 2, EGP2, adenocarcinoma associated antigen, and TROP1. CD326 is a type I transmembrane protein containing six disulfide bridges and one THYRO domain. This cell surface glycosylated 40 kD protein is highly expressed in bone marrow, colon, lung, and most normal epithelial cells and is expressed on carcinomas of gastrointestinal origin. It has been reported that CD326 expression occurs during the early steps of erythrogenesis. CD326 functions as a homotypic calcium-independent cell adhesion molecule and is believed to be involved in carcinogenesis by its ability to induce genes involved in cellular metabolism and proliferation. CD326 antigen is an immunotherapeutic target for the treatment of human carcinomas.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human
Antibody Type: Recombinant
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)
Application Notes: Clone QA20A75 can completely block the binding of clone 9C4 to target cells.
RRID: AB_3105994 (BioLegend Cat. No. 377503)
AB_3105994 (BioLegend Cat. No. 377504)

Antigen Details

Structure: Type I transmembrane protein, contains six disulfide bridges, one THYRO domain, approximate molecular weight 40 kD.
Distribution: Highly expressed in bone marrow, colon, lung, and most normal epithelial cells. Also highly expressed on carcinomas of gastrointestinal origin. Expressed during early erythrogenesis.
Function: Homotypic calcium-independent cell adhesion. CD326 is believed to be involved in carcinogenesis by its ability to induce genes involved in cellular metabolism and proliferation.
Interaction: Human
Cell Type: Embryonic Stem Cells, Epithelial cells
Biology Area: Cell Biology, Immunology, Stem Cells
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: Strnad J, et al. 1989. Cancer Res. 49:314-7.

Munz M, et al. 2004. Oncogene. 23:5748-58.

Rao CG, et al. 2005. Int J Oncol. 27:49-57.
Gene ID: 4072 View all products for this Gene ID
UniProt: View information about CD326 on UniProt.org

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Cell Surface Flow Cytometry Staining Protocol

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Other Formats

View All CD326 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human CD326 (EpCAM) Recombinant Antibody	QA20A75	FC
APC anti-human CD326 (EpCAM) Recombinant Antibody	QA20A75	FC
FITC anti-human CD326 (EpCAM) Recombinant Antibody	QA20A75	FC
PE anti-human CD326 (EpCAM) Recombinant Antibody	QA20A75	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 12/13/2023

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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