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APC anti-human CD74 (Cytoplasmic) Antibody

Pricing & Availability
Clone
Pin.1 (See other available formats)
Regulatory Status
RUO
Other Names
MHC Class II associated invariant chain, invariant chain, Ii, MHC class II chaperone, MIF receptor, DHLAG, HLADG
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
Pin1_APC_CD74_Antibody_1_092017
Human peripheral blood lymphocytes were surface stained with CD19 FITC, fixed and permeabilized, and then stained with CD74 (clone Pin.1) APC (top) or mouse IgG1, κ APC isotype control (bottom).
  • Pin1_APC_CD74_Antibody_1_092017
    Human peripheral blood lymphocytes were surface stained with CD19 FITC, fixed and permeabilized, and then stained with CD74 (clone Pin.1) APC (top) or mouse IgG1, κ APC isotype control (bottom).
  • Pin1_APC_CD74_Antibody_2_092017
Compare all formats See APC spectral data
Cat # Size Price Save
357605 25 tests ¥26,180
357606 100 tests ¥59,840
Description

CD74 is a type II transmembrane glycoprotein also known as MHC class II associated invariant chain, invariant chain, Ii, MHC class II chaperone, and MIF receptor. CD74 exists in four isoforms with molecular masses of 33, 35, 41, and 43 kD, depending on genetic splicing. CD74 is primarily expressed on antigen presenting cells, including B cells, monocytes/macrophages, dendritic cells, and Langerhans cells. It is also expressed by activated T cells and activated endothelial and epithelial cells as well as carcinomas of lung, renal, gastric and thymic origin. The primary function of CD74 is intracellular sorting of MHC class II molecules and regulation of exogenous peptide loading onto MHC class II. It is also involved in the modulation of B cell differentiation and positive selection of CD4+ T cells. It has been reported that CD74 binds MIF (macrophage migration inhibitory factor) and signals through CD44 to regulate innate and adaptive immunity. It is also reported that H. pylori infection occurs through urease B binding of CD74 on gastric epithelial cells, inducing gastric epithelial cell apoptosis, NF-κB activation, and IL-8 production.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Synthetic peptide DQKPVMDDQRDLISNNE, corresponding to amino acids 12-28 of the cytoplasmic tail of human CD74.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
RRID
AB_2716217 (BioLegend Cat. No. 357605)
AB_2716217 (BioLegend Cat. No. 357606)

Antigen Details

Structure
Type II transmembrane glycoprotein, 33, 35, 41, 43 kD
Distribution

B cells and other antigen presenting cells, activated T cells, activated endothelial and epithelial cells, and certain carcinomas

Function
Regulate loading of exogenous peptides onto MHC class II and B cell differentiation, involved in positive selection of CD4+ T cells.
Interaction
Associates with MHC II
Ligand/Receptor
HLA-DR, MIF, H. pylori urease B, associate with CD44
Cell Type
Antigen-presenting cells, B cells, Endothelial cells, Epithelial cells, T cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, MHC Antigens
Antigen References

1. Koch N, et al. 1991. J. Immunol. 147:2643.
2. Wilson KM, et al. 1993. Immunology 79:331.
3. Moldenhauer G, et al. 1999. Immunology 96:473.
4. Shi X, et al. 2006. Immunity 25:595.
5. Beswick EJ, et al. 2006. Infect. Immun. 74:1148.
6. Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules: The CD Markers Wiley-Liss A John Wiley & Sons Inc, Publication.

Gene ID
972 View all products for this Gene ID
UniProt
View information about CD74 on UniProt.org
Go To Top Version: 1    Revision Date: 09/20/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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