APC anti-human IL-9 Antibody, IL-9 MH9A4

Clone: MH9A4 (See other available formats)
Regulatory Status: RUO
Other Names: Interleukin-9, P40, Mast cell growth factor (MCGF), Megakaryoblast growth factor, T cell growth factor-3 (TCGF-3)
Isotype: Mouse IgG2b, κ
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MH9A4_APC_IL-9_Antibody_090619 — PMA and ionomycin-stimulated (6 hours, in the presence of monensin) Th2-polarized human lymphocytes were surface stained with CD3 FITC. Cells were fixed, permeabilized and intracellularly stained with IL-9 (clone MH9A4) APC (left), or mouse IgG2b, κ APC isotype control (right).

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Cat #	Size	Price	Save
507613	25 tests	¥30,140
507614	100 tests	¥95,260

Description

IL-9 is a potent, T cell-derived, T cell growth factor which can also enhance mast cell activity and IL-3- or IL-4- dependent proliferation of bone marrow-derived mast cells. IL-9 synergizes with erythropoietin to promote erythroid colony formation. IL-9 has also been reported to protect human T cells from apoptosis induced by IL-2 withdrawal. IL-9 is upregulated in human eosinophils by TNF-α and IL1-β. IL-9 has been reported to downregulate the oxidative burst in activated human alveolar macropahges and induce TGF-β production. The MH9A4 antibody reacts with human IL-9. The MH9A4 antibody can neutralize the bioactivity of natural or recombinant IL-9.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Baculovirus-expressed, recombinant human IL-9
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Phos™ Perm Buffer in Cell Suspensions Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Red Laser (633 nm)
Application Notes: ELISA Capture²: The purified MH9A4 antibody is useful as the capture antibody in a human IL-9 sandwich ELISA assay, when used in conjunction with the biotinylated MH9D1 antibody as the detecting antibody.
For human IL-9 neutralization assay, the LEAF™ purified MH9D1 antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for neutralization of human IL-9 bioactivity.
RRID: AB_2814435 (BioLegend Cat. No. 507613)
AB_2814435 (BioLegend Cat. No. 507614)

Antigen Details

Structure: Cytokine; 32-39 kD (Mammalian)
Bioactivity: Potentiates production of IgG, IgM, and IgE by IL-4-induced B lymphocytes; regulates granzyme family proteases; enhances proliferation of bone marrow mast cells with IL-3; production of IL-6 by mast cells
Cell Sources: IL-2 activated T_H2 lymphocytes, Hodgkin's lymphoma
Cell Targets: T lymphocytes, erythroid precursors
Receptors: IL-9R
Biology Area: Immunology
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Quesniaux V. 1992. Research Immunology 143:385.
3. Renauld J, et al. 1993. Adv. Immunol. 54:79.
4. Yang Y. 1992. Leuk. Lymphoma 8:441.
Regulation: Upregulated by IL-1 after TCR activation, TNF-α; downregulated by antibodies to IL-2
Gene ID: 3578 View all products for this Gene ID
UniProt: View information about IL-9 on UniProt.org

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Intracellular Flow Cytometry Staining Protocol

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PerCP/Cyanine5.5 anti-human IL-9	MH9A4	ICFC
PE/Cyanine7 anti-human IL-9	MH9A4	ICFC
APC anti-human IL-9	MH9A4	ICFC
TotalSeq™-B1151 anti-human IL-9	MH9A4	ICPG

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 09/06/2019

For Research Use Only. Not for diagnostic or therapeutic use.

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