APC anti-human Siglec-9 Antibody anti-Siglec-9

Clone: K8 (See other available formats)
Regulatory Status: RUO
Other Names: Sialic acid-binding Ig-like lectin-9, CD329
Isotype: Mouse IgG1, κ
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Human peripheral blood granulocytes were stained with Siglec-9 (clone K8) APC (filled histogram) or mouse IgG1, κ APC isotype control (open histogram).

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Cat #	Size	Price	Save
351505	25 tests	¥31,240
351506	100 tests	¥74,360

Description

Siglecs are cell surface receptors belonging to the immunoglobulin superfamily that recognize sugar antigens. The extracellular domain of siglec-9 contains an IgV region, which binds sialic acid, followed by two IgC regions. Siglec 9 and siglec 6-8,10-12 are CD33 (siglec 3) like siglecs, which have two ITIMs in the cytoplasmic tails, suggesting their functional involvement in signal transduction. It is highly expressed on neutrophils and monocytes, and at lower levels on the subpopulations of T and B lymphocytes and NK cells. Siglec-9 plays a role in negative regulation of T cell activation, and it also affects neutrophil apoptosis.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant Siglec-9 fused to Fc region of human IgG
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunofluorescence staining², Western blotting², immunoprecipitation², and ELISA³.
RRID: AB_2564266 (BioLegend Cat. No. 351505)
AB_2564266 (BioLegend Cat. No. 351506)

Antigen Details

Structure: Immunoglobulin superfamily
Distribution: Highly expressed on neutrophils and monocytes, and at lower levels on the subpopulations of T cells and B lymphocytes and NK cells
Ligand/Receptor: Sialic acid
Cell Type: Monocytes, Neutrophils
Biology Area: Costimulatory Molecules, Immunology, Innate Immunity
Molecular Family: CD Molecules, Siglec Molecules
Antigen References: 1. Ikehara Y, et. al. 2004. J. Biol. Chem. 279:43117.
2. von Gunten S, et al. 2005. Blood 106:1423.
Gene ID: 27180 View all products for this Gene ID
UniProt: View information about Siglec-9 on UniProt.org

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Cell Surface Flow Cytometry Staining Protocol

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Other Formats

View All Siglec-9 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human Siglec-9	K8	FC,ELISA,ICC,IP,WB
PE anti-human Siglec-9	K8	FC
APC anti-human Siglec-9	K8	FC
Pacific Blue™ anti-human Siglec-9	K8	FC
FITC anti-human Siglec-9	K8	FC
Alexa Fluor® 647 anti-human Siglec-9	K8	FC
PE/Cyanine7 anti-human Siglec-9	K8	FC
PE/Dazzle™ 594 anti-human Siglec-9	K8	FC
APC/Fire™ 750 anti-human Siglec-9	K8	FC
PerCP/Cyanine5.5 anti-human Siglec-9	K8	FC
TotalSeq™-A1280 anti-human Siglec-9	K8	PG
TotalSeq™-B1280 anti-human Siglec-9	K8	PG
TotalSeq™-C1280 anti-human Siglec-9	K8	PG

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 09/15/2014

For Research Use Only. Not for diagnostic or therapeutic use.

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