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- Clone
- W8B2 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Tissue Non-specific Alkaline Phosphatase, mesenchymal stem cell antigen-1, MSC, W8B2
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
The W8B2 antibody reacts with an antigen, TNAP (Tissue non-specific alkaline phosphatase), that is selectively expressed on bone marrow mesenchymal stem cells, but not on placenta-derived mesenchymal stem cells. It was previously reported that the antibody recognized MSCA-1, mesenchymal stem cell antigen-1. Mesenchymal stem cells or MSCs were originally named as bone marrow stromal cells. They are a population of adult stem cells with a large capacity for self-renewal and multipotency for differentiation into a variety of cell types including osteoblasts, chondrocytes, myocytes, adipocytes, β-pancreatic islets cells and certain neuronal cells. MSCs reside in many tissues, such as bone marrow, placenta, adipose tissue, adult peripheral blood, fetal blood, skin, as well as liver and lung. Besides their plasticity for tissue repair, MSCs also exhibit a powerful immunosuppressive activity and play important role in supporting hematopoiesis.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Reported Reactivity
- African Green, Baboon, Cynomolgus
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Retinoblastoma cell line (Weri-RB-1 cells)
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
- Excitation Laser
-
Red Laser (633 nm)
-
Application References
(PubMed link indicates BioLegend citation) -
- Battula VL, et al. 2009. Haematologica. 94:173.
- Product Citations
-
- RRID
-
AB_2057506 (BioLegend Cat. No. 327308)
Antigen Details
- Distribution
-
Bone marrow mesenchymal stem cells
- Cell Type
- Mesenchymal cells, Mesenchymal Stem Cells
- Biology Area
- Immunology, Stem Cells
- Antigen References
-
1. Battula VL, et al. 2007. Differentiation. 75:279.
- Gene ID
- 249 View all products for this Gene ID
- UniProt
- View information about TNAP on UniProt.org
Related FAQs
Other Formats
View All TNAP Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| PE anti-human TNAP | W8B2 | FC |
| Purified anti-human TNAP | W8B2 | FC |
| APC anti-human TNAP | W8B2 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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PE anti-human TNAP
Human retinoblastoma cell line Weri-RB cells stained W8B2 PE -
Purified anti-human TNAP
Human retinoblastoma cell line WERI-RB-1 stained with purifi... -
APC anti-human TNAP
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