APC anti-mouse CD285 (TLR5) Antibody, CD285, ACT5

Clone: ACT5 (See other available formats)
Regulatory Status: RUO
Other Names: Toll-like receptor 5, TIL3, SLEB1, MELIOS
Isotype: Mouse IgG1, κ
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Product Citations: publications

ACT5_APC_CD285_TLR5_Antibody_032421 — Collagenase-digested C57BL/6 mouse splenocytes were stained with CD11c FITC and anti-mouse CD285 (TLR5) (clone ACT5) APC (left) or mouse IgG1, κ APC isotype control (right).

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Cat #	Size	Price	Save
148105	25 µg	¥39,160
148106	100 µg	¥82,280

Description

CD285, also known as TLR5, is a 98 kD pattern recognition receptor that binds to bacterial flagellin. It is expressed on dendritic cells, classical monocytes and mucosal epithelial cells and is involved in activation of TLR5 induced NF-κB mediated activation of inflammatory-related target genes. Mutations in the TLR5 gene have been linked with susceptibility to systemic lupus erythematosus and Legionnaire’s disease.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: TLR5-transfected Ba/F3 cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications for the relevant formats include: immunoprecipitation of TLR5 from cell lysates^1,2.
RRID: AB_2890695 (BioLegend Cat. No. 148105)
AB_2890695 (BioLegend Cat. No. 148106)

Antigen Details

Structure: Type 1 transmembrane protein, 98 kD
Distribution: Neutrophils, CD11b, Ly6C, classical monocytes, dendritic cells, mucosal epithelium
Interaction: Interacts with MyD88 and TRAF6 leading to NF-κB activation and production of cytokines.
Ligand/Receptor: Flagellin
Cell Type: Dendritic cells, Monocytes, Neutrophils
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules
Antigen References: 1. López-Yglesias AH, et al. 2014. J. Immunol. 192:1587.
2. Burdelya L, et al. 2013. Proc. Natl. Acad. Sci. USA. 110:E1857.
3. Carapau D, et al. 2013. Infect. Immun. 81:4350.
4. Kinnebrew M, et al. 2012. Immunity. 36:276.
5. Sebastiani G, et al. 2000. Genomics. 64:230.
Gene ID: 53791 View all products for this Gene ID
UniProt: View information about CD285 on UniProt.org

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Cell Surface Flow Cytometry Staining Protocol

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Other Formats

View All CD285 Reagents Request Custom Conjugation

Description	Clone	Applications
Alexa Fluor® 647 anti-mouse CD285 (TLR5)	ACT5	FC
Purified anti-mouse CD285 (TLR5)	ACT5	FC,IP
PE anti-mouse CD285 (TLR5) Antibody	ACT5	FC
APC anti-mouse CD285 (TLR5)	ACT5	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 03/04/2021

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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