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APC anti-mouse CD45.1 Antibody

Pricing & Availability
Clone
A20 (See other available formats)
Regulatory Status
RUO
Other Names
T200, Ly-5.1, LCA
Isotype
Mouse (A.SW) IgG2a, κ
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Product Citations
publications
A20_APC_090507
SJL mouse splenocytes stained with A20 APC
  • A20_APC_090507
    SJL mouse splenocytes stained with A20 APC
Compare all formats See APC spectral data
Cat # Size Price Save
110713 25 µg ¥9,680
110714 100 µg ¥23,830
Description

CD45.1 is an alloantigen of CD45, expressed by Ly5.1 bearing mouse strains (e.g., RIII, SJL/J, STS/A, DA). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in mice that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell types. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
SJL mouse thymocytes and splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

The A20 antibody does not react with leukocytes or mouse cells expressing the CD45.2 alloantigen. Additional reported applications (for relevant formats of this clone) include: immunoprecipitation3, in vitro blocking of B cell responses1,2, immunohistochemical staining of frozen sections: OCT embedded7 and acetone-fixed4-6 (direct immunofluorescence detection with fluorochrome conjugated A20 was used in (5) and (6)).

Application References

(PubMed link indicates BioLegend citation)
  1. Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
  2. Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
  3. Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
  4. Suzuki K, et al. 2000. Immunity 13:691. (IHC-F)
  5. Werner N, et al. 2002. Arterioscler. Thromb. Vasc. Biol. 22:1567. (IHC-F)
  6. Lessner SM, et al. 2002. Am. J. Pathol. 160:2145. (FC, IHC-F)
  7. Chen CC, et al. 2005. P. Natl. Acad. Sci. USA 102:11408 (IHC-F)
  8. Pascal V, et al. 2007. J. Immunol. 179:1751. (FC)
  9. Mende I, et al. 2006. Blood 107:1383. (IHC-F, FC)
  10. Phan TG, et al. 2007. Nature Immunol. 8:992. (FC)
  11. Wither DR, et al. 2009. J. Immunol. 183:5079. PubMed
  12. Pascal V, et al.2007. J. Immunol. 179:1751. PubMed
  13. Lee SW, et al. 2009. J. Immunol. 182:6753. PubMed
  14. Takada K, et al. 2009. J. Exp Med. 206:2253. PubMed
  15. Beamer CA, et al. 2007. Am. J. Respir. Cell. Mol. Biol. 37:729. (FC) PubMed
  16. Li LX, et al. 2010. J. Immunol. 184:1728. PubMed
  17. Hosoi A, et al. 2008. Cancer Res. 68:3941. (FC) PubMed
  18. Kenna TJ, et al. 2008. Blood 111:2091. PubMed
  19. Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
Product Citations
  1. Sandu I, et al. 2020. Nat Commun. 11:4454. PubMed
  2. Behr FM, et al. 2021. Eur J Immunol. 51:151. PubMed
  3. Thomas R, et al. 2021. Immunology. 163:478. PubMed
  4. Lehmkuhl P, et al. 2021. Cell Mol Immunol. 18:1677. PubMed
  5. Cohen K, et al. 2021. Cell Rep. 37:110026. PubMed
  6. Durgin JS, et al. 2022. Mol Ther. 30:1201. PubMed
  7. Kragten NAM, et al. 2022. Eur J Immunol. 52:389. PubMed
  8. Parga-Vidal L, et al. 2022. Eur J Immunol. 52:1095. PubMed
  9. Iberg CA, et al. 2022. Cell Rep. 39:110657. PubMed
  10. Kraemer AN, et al. 2022. Front Immunol. 13:933191. PubMed
  11. Tatsumi N, et al. 2022. STAR Protoc. 3:101845. PubMed
  12. Giannou AD, et al. 2023. Immunity. 56:125. PubMed
  13. Scherer S, et al. 2023. Nat Immunol. 24:501. PubMed
  14. Harsha Krovi S, et al. 2020. Nat Commun. 4.790277778. PubMed
  15. Hou X, et al. 2020. Cell Reports. 28(1):172-189.e7.. PubMed
  16. Yi C, et al. 2023. J Neuroinflammation. 20:45. PubMed
  17. Nixon BG, et al. 2023. STAR Protoc. 4:102185. PubMed
  18. Oh J, et al. 2023. Nat Commun. 14:3278. PubMed
  19. Stanzione M, et al. 2022. Sci Adv. 8:eabn1229. PubMed
  20. Gern BH, et al. 2021. Cell Host Microbe. 29(4):594-606.e6. PubMed
  21. Hewitson JP, et al. 2020. J Immunol. 204:2949. PubMed
  22. Pessoa Rodrigues C, et al. 2021. Sci Adv. 7:. PubMed
  23. Tan X, et al. 2016. PLoS One. 11: 0160985. PubMed
  24. Sano T, et al. 2021. Cell Reports. 36:109608. PubMed
  25. Lancaster JN, et al. 2019. Nat Commun. 10:2220. PubMed
  26. Bangs DJ, et al. 2022. Cell Rep. 38:110266. PubMed
  27. Klarquist J, et al. 2021. Cell Rep. 36:109591. PubMed
  28. Bhattacharjee S, et al. 2019. Cell Rep. 28:231. PubMed
  29. Chen J et al. 2018. Cell reports. 25(12):3393-3404 . PubMed
  30. Inoue D, et al. 2021. Nat Genet. 53:707. PubMed
  31. Zong L, et al. 2021. NPJ Aging Mech Dis. 7:25. PubMed
  32. Okuniewska M, et al. 2021. Cell Reports. 36(2):109368. PubMed
  33. Brooks JF, et al. 2020. Cytotherapy. 22:436. PubMed
  34. Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed
  35. Misumi I et al. 2019. Cell Rep. 27(2):514-524 . PubMed
  36. Bhattacherjee A, et al. 2021. Mol Neurodegener. 16:19. PubMed
  37. Wang C, et al. 2021. Cell Rep. 37:110021. PubMed
  38. Pawaria S, et al. 2020. Arthritis Rheumatol. 72:359. PubMed
  39. Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed
  40. Zhuang Z, et al. 2021. J Exp Med. 218:00:00. PubMed
  41. Brocq M, et al. 2014. J Immunol. 192:6120. PubMed
  42. Yoshimi A, et al. 2019. Nature. 574:273. PubMed
  43. Paiva RA, et al. 2021. Cell Reports. 35(2):108967. PubMed
  44. García-Bonilla M, et al. 2020. Stem Cell Res Ther. 11:121. PubMed
  45. Ramos CV, et al. 2020. Cell Reports. 32(3):107910. PubMed
  46. Marchingo JM, et al. 2020. eLife. 9:e53725.. PubMed
  47. Mansouri S, et al. 2021. J Immunol. 206:2233. PubMed
  48. Varol D et al. 2017. Immunity. 46(6):1030-1044 . PubMed
  49. Kaplan BLF et al. 2018. Current protocols in toxicology. 75:11:00 25. PubMed
  50. Xu L et al. 2017. Immunity. 47(3):538-551 . PubMed
  51. Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed
  52. Li J, et al. 2020. Elife. 9:00. PubMed
  53. Sinclair LV et al. 2019. Elife. 8 pii: e44210. PubMed
  54. Chen X, et al. 2021. Cell Rep. 37:109991. PubMed
  55. van Montfoort N, et al. 2018. Cell. 175:1744. PubMed
  56. Trefzer A, et al. 2021. Cell Reports. 34(6):108748. PubMed
  57. Wu J et al. 2019. Immunity. 50(5):1218-1231 . PubMed
  58. Frost JN, et al. 2021. Med (N Y). 2:164. PubMed
  59. Port JR, et al. 2020. J Virol. 94:. PubMed
  60. Bates PD, et al. 2021. Front Immunol. 12:668307. PubMed
  61. Korrer M, Routes J 2014. PLoS One. 9:91370. PubMed
  62. Wang L, et al. 2014. Proc Natl Acad Sci U S A. 111:3146. PubMed
  63. Landon J Edgar et al. 2018. Cell chemical biology. 26(1):131-136 . PubMed
  64. Taylor D, et al. 2022. Front Immunol. 13:936129. PubMed
  65. Sadik A, et al. 2020. Cell. 1252:182. PubMed
  66. Xu P, et al. 2020. Cancer Immunol Res. 8:1193. PubMed
  67. Fang F, et al. 2021. Cell Rep. 37:109981. PubMed
  68. Mansouri S, et al. 2020. Mucosal Immunol. 0.954861111. PubMed
  69. Muri J, et al. 2020. eLife. 9:e53627.. PubMed
  70. Xueyang Yu et al. 2017. Immunity. 47(5):903-912 . PubMed
  71. Sato K, et al. 2017. J Immunol. 10.4049/jimmunol.1602005. PubMed
  72. Wu J, et al. 2021. STAR Protoc. 2:101022. PubMed
  73. Kissiov DU, et al. 2022. Elife. 11:. PubMed
  74. Alexander Mildner et al. 2017. Immunity. 46(5):849-862 . PubMed
  75. Chen C, et al. 2020. Cell Rep. 2136:30. PubMed
  76. Ocaña-Morgner C, et al. 2013. J Immunol. 190:5545. PubMed
  77. Sun Y, et al. 2020. J Immunol. 205:2649. PubMed
  78. Schaffert S, et al. 2015. J Immunol. 195: 1470-1479. PubMed
  79. Sun Y, et al. 2022. iScience. 25:104846. PubMed
  80. Endo-Umeda K, et al. 2021. Sci Rep. 11:22595. PubMed
  81. Kohn M, et al. 2021. Front Immunol. 626627:12. PubMed
  82. Martins R, et al. 2016. Nat Immunol. 17:1361-1372. PubMed
  83. Akane K, et al. 2016. Proc Natl Acad Sci U S A. 113: 2460 - 2465. PubMed
  84. Hu Z, et al. 2013. J Immunol. 191:312. PubMed
  85. Lu Y, et al. 2018. Cancer Cell. 33:1048. PubMed
  86. Dourcy M, et al. 2020. Mucosal Immunol. 13:799. PubMed
  87. Mansouri S, et al. 2021. J Immunol. 206:2233. PubMed
  88. Alterauge D, et al. 2020. Cell Rep. 33:108232. PubMed
  89. Patterson DG, et al. 2021. J Immunol. 207:1798. PubMed
  90. Pessoa Rodrigues C, et al. 2020. Sci Adv. 6:eaaz4815. PubMed
RRID
AB_313502 (BioLegend Cat. No. 110713)
AB_313502 (BioLegend Cat. No. 110714)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets of the CD45.1 strain of mice

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45.1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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