APC anti-mouse CD70 Antibody CD70 FR70

Clone: FR70 (See other available formats)
Regulatory Status: RUO
Other Names: Ki-24, CD27-ligand, TNFSF7
Isotype: Rat IgG2b, κ
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Product Citations: publications

FR70_APC_CD70_Antibody_091018 — B cell lymphoma cell line, A20, was stained with CD70 (clone FR70) APC (filled histogram) or rat IgG2b, κ isotype APC control (open histogram).

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Cat #	Size	Price	Save
104609	25 µg	¥24,860
104610	100 µg	¥47,520

Description

CD70 is a type II transmembrane protein. It is a member of the TNF superfamily, expressed on activated B cells, activated dendritic cells, and some activated T cells (low levels). CD70 interacts with CD27 to promote T-B cell cross-stimulation and co-stimulate B cell proliferation and immunoglobulin production. Cells expressing CD70 can co-stimulate T cell proliferation and enhance the production of cytokines. The FR70 antibody blocks CD70 binding to CD27.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: BALB/c mouse B lymphoma A20.2J
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation¹, in vitro blocking of CD70-CD27 binding and inhibition of T and NK cell responses^1-3 , and immunohistochemistry⁴ of aceton-fixed frozen sections. For the most successful immunofluorescent staining results, it may be necessary to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 104605/104606) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 104604) or biotinylated anti-rat IgG (Cat. No. 405402) second step, followed by SAv-PE (Cat. No. 405204)). To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 (Cat. No. 101301/101302), is recommended prior to immunofluorescent staining. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 104608).
RRID: AB_2750464 (BioLegend Cat. No. 104609)
AB_2750464 (BioLegend Cat. No. 104610)

Antigen Details

Structure: TNF/NGF superfamily, 30-33 kD
Distribution: Activated B cells and some activated T cells
Function: T-B cell cross-stimulation
Ligand/Receptor: CD27
Cell Type: B cells, T cells
Biology Area: Immunology, Costimulatory Molecules
Molecular Family: CD Molecules
Antigen References: 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Tesselaar K, et al. 1997. J. Immunol. 159:4959.
3. Akiba H, et al. 1999. J. Immunol. 162:7058.
Gene ID: 21948 View all products for this Gene ID
UniProt: View information about CD70 on UniProt.org

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Other Formats

View All CD70 Reagents Request Custom Conjugation

Description	Clone	Applications
Biotin anti-mouse CD70	FR70	FC
PE anti-mouse CD70	FR70	FC
PE/Cyanine7 anti-mouse CD70	FR70	FC
PerCP/Cyanine5.5 anti-mouse CD70	FR70	FC
APC anti-mouse CD70	FR70	FC

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 09/10/2018

For Research Use Only. Not for diagnostic or therapeutic use.

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