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APC/Cyanine7 anti-human CD39 Antibody

Pricing & Availability
Clone
A1 (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
gp80, E-ATPDase, NTPDase-1, ecto-apyrase, Ec3.6.1.5
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A1_APCCyanine7_CD39_Antibody_013019_updated.png
Human peripheral blood lymphocytes were stained with CD19 FITC and CD39 (clone A1) APC/Cyanine7 (left) or Mouse IgG1, κ APC/Cyanine7 isotype control (right).
  • A1_APCCyanine7_CD39_Antibody_013019_updated.png
    Human peripheral blood lymphocytes were stained with CD19 FITC and CD39 (clone A1) APC/Cyanine7 (left) or Mouse IgG1, κ APC/Cyanine7 isotype control (right).
Compare all formats See APC/Cyanine7 spectral data
Cat # Size Price Save
328225 25 tests ¥36,300
328226 100 tests ¥74,800
Description

Human CD39 is an integral membrane protein with two transmembrane domains. It exists as a homotetramer. Expression of CD39 is found on activated lymphocytes, a subset of T cells and B cells, and dendritic cells with weak staining on monocytes and granulocytes. CD39 and CD73 have been found on regulatory T cells, specifically the effector/memory like T cells. CD39 can hydrolyze both nucleoside triphosphates and diphosphates. CD39 is the dominant ecto nucleotidase of vascular and placental trophoblastic tissues and appears to modulate the functional expression of type 2 purinergic (P2) G protein coupled receptors (GPCRs). CD39 has intrinsic ecto-ATPase activity. Expression of CD39 is induced on T cells and increased on B cells as a late activation antigen.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
PHA activated human lymphocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

The A1 antibody binds to the human CD39 cell surface antigen and has been shown to block MHC independent target cell recognition by hapten-specific CTL. Additional reported applications (for the relevant formats) include: in vitro CD39 blockade3,  immunofluorescence4, immunohistochemistry6, and spatial biology (IBEX)7,8. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for blocking assays (contact our custom solutions team).

Application References

(PubMed link indicates BioLegend citation)
  1. Aversa GG, et al. 1988. Transplant. P. 20:4952.
  2. Aversa GG, et al. 1989. Transplant. P. 21:34950.
  3. Borsellino G, et al. 2007. Blood. 110:1225. (Block)
  4. Stockl J, et al. 2001. J. Immunol. 167:2724. (IF)
  5. Sestak K, et al. 2007. Vet. Immunol. Immunopathol. 119:21.
  6. Lyck L, et al. 2008. J. Histochem. Cytochem. 56:201. (IHC)
  7. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  8. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Huang B, et al. 2020. Cell. 179(5):1160-1176.e24.. PubMed
  2. Sade–Feldman M, et al. 2018. Cell. 175:998. PubMed
  3. Tiberti S, et al. 2022. Nat Commun. 13:6752. PubMed
RRID
AB_2571980 (BioLegend Cat. No. 328225)
AB_2571980 (BioLegend Cat. No. 328226)

Antigen Details

Distribution

Activated lymphocytes, and also on a subset of T cells, regulatory T cells, B cells, and dendritic cells.

Cell Type
B cells, Dendritic cells, Lymphocytes, T cells, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Gene ID
953 View all products for this Gene ID
UniProt
View information about CD39 on UniProt.org
Go To Top Version: 2    Revision Date: 01/30/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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