APC/Fire™ 750 anti-GATA3 Antibody, GATA3, W19195B

Clone: W19195B (See other available formats)
Regulatory Status: RUO
Other Names: GATA-binding factor 3, trans-acting T-cell-specific transcription factor GATA-3
Isotype: Rat IgG2a, κ
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Product Citations: publications

A. W19195B_APC-FIRE-750_GATA3_ICFC-1_122723 — Murine Th2 cell line D10.G4.1 was fixed and permeabilized with True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401), and intracellularly stained with anti-GATA3 (clone W19195B) APC/Fire™ 750 (filled histogram), or rat IgG2a, κ APC/Fire™ 750 isotype control (open histogram).

Compare all formats See APC/Fire™ 750 spectral data

Cat #	Size	Price	Save
386905	25 tests	¥40,260
386906	100 tests	¥94,380

Description

GATA3 functions as a major regulator of T helper 2 (Th2) cell differentiation in immune cells. GATA3 expression is upregulated through IL-4 receptor signaling or the Notch-mediated pathway, which results in production of IL-4, IL-5, and IL-13 cytokines. This is responsible for the preferential differentiation of Th2-cells. GATA3 has been shown to regulate early developmental processes of T cells, such as T cell commitment, positive selection, and post-commitment CD4+ T cell survival. In the mammary gland, GATA3 plays an important role in differentiation and commitment of luminal epithelial cells. In a mouse model, exogenous expression of GATA3 in undifferentiated breast carcinoma cells induces differentiation and suppresses spreading of the tumor cells, suggesting that GATA3 is involved in preventing malignancy of breast cancer. GATA3 has been reported to be essential in the development of a variety of normal tissues. Defects in GATA3 result in hypoparathyroidism, sensorineural deafness, and renal dysplasia (HDR syndrome).

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Partial human GATA3 recombinant protein (1-258 aa)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µL per 10^6 cells in 100 µL volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 774 nm.
Excitation Laser: Red Laser (633 nm)
Application Notes: Clone W19195B does not block 16E10A23, TWAJ, or L50-823.
RRID: AB_3097419 (BioLegend Cat. No. 386905)
AB_3097419 (BioLegend Cat. No. 386906)

Antigen Details

Structure: 48 kD protein containing two GATA-type zinc finger domains responsible for DNA binding and two trans-activating domains at N-terminus
Distribution: Nucleus
Function: GATA3, a transcriptional activator, binds to a consensus DNA sequence 5'-(A/T) GATA(A/G)-3' in the promoter region of genes, regulates T cell development, TH2 cell differentiation, and luminal cell proliferation
Interaction: Interacts with Smad3, FOG1, FOG2, and PU.1
Biology Area: Cell Biology, Chromatin Remodeling/Epigenetics, Immunology, Signal Transduction, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: Yagi R, et al. 2011. Int Immunol. 23:415-20.

Chou J, et al. 2010. J Cell Physiol. 222:42-9.

Ho IC, et al. 2009. Nat Rev Immunol. 9:125-35.

Rothenberg EV, et al. 2008. Semin Immunol. 20:236-46.

Kouros-Mehr H, et al. 2008. Curr Opin Cell Biol. 20:164-70.

Van Esch H, et al. 2000. Nature. 406:419-22.
Gene ID: 2625 View all products for this Gene ID
UniProt: View information about GATA3 on UniProt.org

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Other Formats

View All GATA3 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-GATA3	W19195B	ICFC
PE anti-GATA3	W19195B	ICFC
APC/Fire™ 750 anti-GATA3	W19195B	ICFC
APC anti-GATA3	W19195B	ICFC
PerCP/Cyanine5.5 anti-GATA3	W19195B	ICFC
Brilliant Violet 421™ anti-GATA3	W19195B	ICFC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 12/27/2023

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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