APC/Fire 750 anti-human CD20 Antibody anti-CD20

Clone: 2H7 (See other available formats)
Regulatory Status: RUO
Workshop: IV B201
Other Names: B1, Bp35
Isotype: Mouse IgG2b, κ
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Product Citations: publications

2H7_APCFire750_CD20_Antibody_1_030816 — Human peripheral blood lymphocytes were stained with CD3 PE and CD20 (clone 2H7) APC/Fire™ 750 (top) or mouse IgG2b, κ APC/Fire™ 750 isotype control (bottom).

Compare all formats See APC/Fire™ 750 spectral data

Cat #	Size	Price	Save
302357	25 tests	¥34,980
302358	100 tests	¥76,340

Description

CD20 is a 33-37 kD, four transmembrane spanning protein, also known as B1 and Bp35. CD20 is expressed on pre-B-cells, resting and activated B cells (not plasma cells), some follicular dendritic cells, and at low levels on a T cell subset. CD20 is heavily phosphorylated on activated B cells and malignant B cells. Homo-oligomeric complexes of CD20 are thought to form Ca²⁺ conductive ion channels in the plasma membrane of B cells. The CD20 molecule is involved in B-cell activation and is associated with various Src family kinases (Lyn, Lck, Fyn). It exists in a complex with MHC class I and II, CD53, CD81, and CD82.

Product Details

Technical data sheet

Product Details

Verified Reactivity

Human, Cynomolgus, Rhesus

Reported Reactivity

Baboon, Capuchin Monkey, Chimpanzee, Pigtailed Macaque, Squirrel Monkey

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Human tonsillar B cells

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.

* APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 774 nm.

Application Notes

The epitope recognized by clone 2H7 has been mapped to the sequence YNCEPANPSEKNSPST which lies in the large extracellular loop of human CD20. Additional reported applications (for the relevant formats) include: immunoprecipitation⁴ and immunohistochemical staining of acetone-fixed frozen sections⁵.

Application References (PubMed link indicates BioLegend citation)

Schlossman S, et al. 1995. Leucocyte Typing V. Oxford University Press. New York.
Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
McMichael A, et al. Eds. 1987. Leucocyte Typing III Oxford University Press. New York.
Polyak MJ, et al. 2002. Blood 99:3256. (IP)
Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)

Product Citations

Gao Q, et al. 2022. Curr Protoc. 2:e503. PubMed
Toriyama M, et al. 2023. Front Mol Biosci. 10:1149828. PubMed
Lindesmith LC, et al. 2020. Cell Mol Gastroenterol Hepatol. 0.586805556. PubMed
Turner JS, et al. 2021. Nature. 596:109. PubMed
Madsen A, et al. 2020. Immunity. 53:852. PubMed
Turner JS, et al. 2020. Nature. 586:127. PubMed
Kunishita Y, et al. 2020. Front Immunol. 11:98. PubMed
Turner JS, et al. 2021. Nature. 595:421. PubMed

RRID

AB_2572125 (BioLegend Cat. No. 302357)
AB_2572125 (BioLegend Cat. No. 302358)

Antigen Details

Structure: Four transmembrane protein (TM4SF), heavily phosphorylated after activation, 33-37 kD
Distribution: B cell, T cell subsets
Function: B cell activation
Ligand/Receptor: Src family tyrosine kinases, MHC class I, II, CD53, CD81, CD82
Cell Type: B cells, T cells
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Hultin L, et al. 1993. Cytometry 14:196.
2. Tedder T, et al. 1994. Immunol. Today 15:450.
Gene ID: 931 View all products for this Gene ID
UniProt: View information about CD20 on UniProt.org

Documentation

Reviews

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

Related Products

Description	Clone	Applications

Related Pages & Pathways

Pathways

B cell Development and Activation

Related FAQs

What is the difference between two anti human CD20 clones 2H7 and 1412?: For clone 1412: This clone specifically recognizes cytoplasmic domain of CD20 and thus can only be used for intracellular flow cytometry. In this instance you will need to include the fixation and permeabilization steps. Please follow the intracellular flow cytometry staining protocol.

For clone 2H7: This clone is ok for regular surface staining for CD20 and there is no need for any fixation and permeabilization steps.

Our technical protocols can be found here.

Other Formats

View All CD20 Reagents Request Custom Conjugation

Description	Clone	Applications
APC anti-human CD20	2H7	FC
FITC anti-human CD20	2H7	FC
PE anti-human CD20	2H7	FC
PE/Cyanine5 anti-human CD20	2H7	FC
Purified anti-human CD20	2H7	FC,CyTOF®,IHC-F,IP
APC/Cyanine7 anti-human CD20	2H7	FC
PE/Cyanine7 anti-human CD20	2H7	FC
Alexa Fluor® 488 anti-human CD20	2H7	FC,IHC-F
Alexa Fluor® 647 anti-human CD20	2H7	FC,IHC-F
Pacific Blue™ anti-human CD20	2H7	FC
Alexa Fluor® 700 anti-human CD20	2H7	FC
PerCP anti-human CD20	2H7	FC
PerCP/Cyanine5.5 anti-human CD20	2H7	FC
Brilliant Violet 421™ anti-human CD20	2H7	FC,IHC-F
Brilliant Violet 570™ anti-human CD20	2H7	FC
Brilliant Violet 605™ anti-human CD20	2H7	FC
Brilliant Violet 650™ anti-human CD20	2H7	FC
Brilliant Violet 785™ anti-human CD20	2H7	FC
Brilliant Violet 510™ anti-human CD20	2H7	FC
Brilliant Violet 711™ anti-human CD20	2H7	FC
Purified anti-human CD20 (Maxpar® Ready)	2H7	FC,CyTOF®
PE/Dazzle™ 594 anti-human CD20	2H7	FC
Biotin anti-human CD20	2H7	FC
APC/Fire™ 750 anti-human CD20	2H7	FC
Alexa Fluor® 594 anti-human CD20	2H7	ICC,IHC-F
FITC anti-human CD20	2H7	FC
Pacific Blue™ anti-human CD20	2H7	FC
APC anti-human CD20	2H7	FC
PE/Cyanine7 anti-human CD20	2H7	FC
TotalSeq™-A0100 anti-human CD20	2H7	PG
TotalSeq™-B0100 anti-human CD20	2H7	PG
TotalSeq™-C0100 anti-human CD20	2H7	PG
PerCP/Cyanine5.5 anti-human CD20	2H7	FC
Spark NIR™ 685 anti-human CD20	2H7	FC
Spark YG™ 593 anti-human CD20	2H7	FC
GMP FITC anti-human CD20	2H7	FC
TotalSeq™-D0100 anti-human CD20	2H7	PG
GMP APC anti-human CD20	2H7	FC
Spark Violet™ 500 anti-human CD20	2H7	FC
GMP Pacific Blue™ anti-human CD20	2H7	FC
GMP PerCP/Cyanine5.5 anti-human CD20	2H7	FC
Spark Violet™ 538 anti-human CD20	2H7	FC
APC/Fire™ 750 anti-human CD20	2H7	FC
PE anti-human CD20	2H7	FC
GMP PE/Cyanine7 anti-human CD20	2H7	FC
Spark Blue™ 515 anti-human CD20	2H7	FC
GMP APC/Fire™ 750 anti-human CD20	2H7	FC
GMP PE anti-human CD20	2H7	FC
Spark Blue™ 550 anti-human CD20 (Flexi-Fluor™)	2H7	FC
Spark Blue™ 574 anti-human CD20 (Flexi-Fluor™)	2H7	FC
Spark Red™ 718 anti-human CD20 (Flexi-Fluor™)	2H7	FC
APC/Fire™ 810 anti-human CD20	2H7	FC
Alexa Fluor® 660 anti-human CD20	2H7	FC

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 03/15/2016

For Research Use Only. Not for diagnostic or therapeutic use.

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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