APC/Fire 750 anti-human CD25 Antibody anti-CD25

Clone: M-A251 (See other available formats)
Regulatory Status: RUO
Workshop: IV A053
Other Names: IL-2 receptor α chain, Low affinity IL-2R, IL-2Rα chain
Isotype: Mouse IgG1, κ
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Product Citations: publications

MdashA251_APCFire750_CD25_Antibody_042716 — PHA-stimulated (3 days) human peripheral blood lymphocytes were stained with CD25 (clone M-A251) APC/Fire™ 750 (filled histogram) or mouse IgG1, κ APC/Fire™ 750 isotype control (open histogram).

Compare all formats See APC/Fire™ 750 spectral data

Cat #	Size	Price	Save
356135	25 tests	¥33,000
356136	100 tests	¥70,180

Description

CD25 is a 55 kD type I transmembrane glycoprotein also known as low affinity IL-2 receptor α chain or Tac. It is expressed on progenitor lymphocytes, activated T and B cells, and activated monocytes/macrophages. CD25 is also expressed on a subset of non-stimulated CD4⁺ T cells termed T regulatory cells. Soluble CD25/IL-2Rα is produced as a consequence of lymphocyte stimulation and is found in biological fluids following inflammatory responses. CD25 associates with IL-2 receptor β (CD122) and common γ (CD132) chains to form a high affinity IL-2R complex.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human
Reported Reactivity: Baboon, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human PHA-induced lymphocyte cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.

* APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 774 nm.
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraformaldehyde fixed frozen sections¹ and spatial biology (IBEX)^2,3.

The CD25 molecule reveals three epitope regions: A, B, and C. M-A251 antibody recognizes epitope region B. Unlike other CD25 antibody clones, M-A251 can detect CD25 after fixation with paraformaldehyde.
RRID: AB_2616723 (BioLegend Cat. No. 356135)
AB_2616723 (BioLegend Cat. No. 356136)

Antigen Details

Structure: Type I transmembrane glycoprotein, 55 kD; low-affinity IL-2 receptor α chain
Distribution: Activated T and B cells, monocytes/macrophages, Tregs
Interaction: Associates with IL-2Rβ/CD122 and IL-2Rγ/CD132 receptor chains to form a high-affinity IL-2R complex
Ligand/Receptor: IL-2
Cell Type: B cells, Macrophages, Monocytes, T cells, Tregs
Biology Area: Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: 1. Knapp W, et al. 1989. Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press.
2. Schlossman S, et al. 1995. Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press.
3. Barclay N, et al. 1997. The Leukocyte Antigen FactsBook. Academic Press Inc.
4. Taniguchi T and Minami Y. et al. 1993. Cell 73:5.
5. Waldmann T. 1991. J. Biol. Chem. 266:2681.
Gene ID: 3559 View all products for this Gene ID
UniProt: View information about CD25 on UniProt.org

Documentation

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PE anti-human CD25	M-A251	FC
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PerCP/Cyanine5.5 anti-human CD25	M-A251	FC
Brilliant Violet 421™ anti-human CD25	M-A251	FC
Alexa Fluor® 488 anti-human CD25	M-A251	FC
Alexa Fluor® 700 anti-human CD25	M-A251	FC
Brilliant Violet 510™ anti-human CD25	M-A251	FC
PE/Dazzle™ 594 anti-human CD25	M-A251	FC
Biotin anti-human CD25	M-A251	FC
Alexa Fluor® 647 anti-human CD25	M-A251	FC,SB
Pacific Blue™ anti-human CD25	M-A251	FC
PerCP anti-human CD25	M-A251	FC
APC/Fire™ 750 anti-human CD25	M-A251	FC
Brilliant Violet 711™ anti-human CD25	M-A251	FC
Brilliant Violet 785™ anti-human CD25	M-A251	FC
Brilliant Violet 605™ anti-human CD25	M-A251	FC
KIRAVIA Blue 520™ anti-human CD25	M-A251	FC
PE/Fire™ 700 anti-human CD25	M-A251	FC
APC/Fire™ 810 anti-human CD25	M-A251	FC
Spark NIR™ 685 anti-human CD25 Antibody	M-A251	FC
Spark YG™ 581 anti-human CD25	M-A251	FC
PE/Fire™ 640 anti-human CD25 Antibody	M-A251	FC
PE anti-human CD25	M-A251	FC
PerCP/Cyanine5.5 anti-human CD25	M-A251	FC
APC/Fire™ 750 anti-human CD25	M-A251	FC
PE/Cyanine7 anti-human CD25	M-A251	FC
APC anti-human CD25	M-A251	FC
PE/Cyanine5 anti-human CD25	M-A251	FC
FITC anti-human CD25	M-A251	FC
Spark Red™ 718 anti-human CD25	M-A251	FC
GMP PE anti-human CD25	M-A251	FC
GMP PE/Cyanine7 anti-human CD25	M-A251	FC
PerCP/Fire™ 780 anti-human CD25	M-A251	FC
PE/Fire™ 744 anti-human CD25	M-A251	FC
PerCP/Fire™ 806 anti-human CD25	M-A251	FC
Brilliant Violet 650™ anti-human CD25	M-A251	FC
Brilliant Violet 750™ anti-human CD25	M-A251	FC

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 04/27/2016

For Research Use Only. Not for diagnostic or therapeutic use.

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