APC/Fire™ 750 anti-mouse CD20 Antibody, CD20, SA271G2

Clone: SA271G2 (See other available formats)
Regulatory Status: RUO
Other Names: Ms4a1, Ly-44
Isotype: Rat IgG2b, κ
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Product Citations: publications

SA271G2_APCF750_CD20_Antibody_030122 — C57BL/6 mouse splenocytes were stained with anti-mouse CD45R/B220 FITC and anti-mouse CD20 (clone SA271G2) APC/Fire™ 750 (left) or rat IgG2b, κ APC/Fire™ 750 isotype control (right).

Compare all formats See APC/Fire™ 750 spectral data

Cat #	Size	Price	Save
152117	25 µg	¥34,980
152118	100 µg	¥84,040

Description

CD20 is a 33-37 kD protein, a member of the MS4A family, with four transmembrane spanning regions that present as a homo-oligomeric complexes in the cell surface when associating with MHC class I and II, CD53, CD81, and CD82. CD20 is expressed on B cells and a subset of T cells, but not on plasma cells. CD20 regulates B-cell activation and proliferation. Its ligation promotes transmembrane Ca²⁺ trafficking. CD20 is an important therapeutic target in the treatment of B cell lymphomas and leukemias.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse CD20 transfected cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 774 nm.
Excitation Laser: Red Laser (633 nm)
RRID: AB_2910309 (BioLegend Cat. No. 152117)
AB_2910309 (BioLegend Cat. No. 152118)

Antigen Details

Structure: Member of the MS4A family, four transmembrane spanning regions, three isoforms of 37, 35, and 37 kD, depending on the phosphorylation degree.
Distribution: B cells and a subset of T cells
Function: Regulates B-cell activation and proliferation
Interaction: Associates with MHC class I and II, CD53, CD81, and CD82
Cell Type: B cells, T cells
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: Morsy DE, et al. 2013. J Immunol. 191:3112-8.

Lund FE and Randall TD. 2010. Nat Rev Immunol. 10:236-47.

Beers SA, et al. 2010. Blood. 115:5191-201.

Kuijpers TW, et al. 2010. J Clin Invest. 120:214-22.
Gene ID: 12482 View all products for this Gene ID
UniProt: View information about CD20 on UniProt.org

Documentation

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

Related Products

Description	Clone	Applications

Related Pages & Pathways

Pathways

B cell Development and Activation

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD20 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-mouse CD20	SA271G2	FC,Depletion
Ultra-LEAF™ Purified anti-mouse CD20	SA271G2	FC,Depletion
PE anti-mouse CD20	SA271G2	FC
APC anti-mouse CD20	SA271G2	FC
PE/Dazzle™ 594 anti-mouse CD20 Antibody	SA271G2	FC
FITC anti-mouse CD20	SA271G2	FC
PE/Cyanine5 anti-mouse CD20	SA271G2	FC
APC/Fire™ 750 anti-mouse CD20	SA271G2	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 03/01/2022

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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