APC/Fire™ 810 anti-mouse Ly-6C Antibody, Ly-6C, HK1.4

Clone: HK1.4 (See other available formats)
Regulatory Status: RUO
Other Names: Lymphocyte antigen 6 complex, locus C
Isotype: Rat IgG2c, κ
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C57BL/6 mouse splenocytes were stained with anti-mouse CD8a PE and anti-mouse Ly-6C (clone HK1.4) APC/Fire™ 810 (left) or rat IgG2a, κ APC/Fire™ 810 isotype control (right).

Compare all formats See APC/Fire™ 810 spectral data

Cat #	Size	Price	Save
128055	25 µg	¥37,620
128056	100 µg	¥78,760

Description

Most hematopoietic cells express one or more members of Ly-6 family. The expression of Ly-6 varies with development stage and activation. Ly-6C is a 14-17 kD GPI-linked surface protein expressed on mouse monocyte/macrophage cells, endothelial cells, neutrophils, and some T cell subsets. Ly-6C is reported to be an indicator of memory CD8⁺ T cells.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: L3 cloned CTL cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 810 under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* APC/Fire™ 810 has a maximum excitation of 650 nm and a maximum emission of 810 nm.

Excessive exposure to light, and commonly used fixation, permeabilization buffers can affect APC/Fire™ 810 fluorescence signal intensity and spread. Please keep conjugates protected from light exposure. For more information and representative data, visit our Fire Dyes page.
Excitation Laser: Red Laser (633 nm)
Application Notes: Clone HK1.4 does not block the binding of clone RB6-8C5⁸.

Additional reported applications (for relevant formats of this clone) include: in vitro activation of T cells^1-3 and immunohistochemistry of frozen sections⁴.
RRID: AB_2910291 (BioLegend Cat. No. 128055)
AB_2910291 (BioLegend Cat. No. 128056)

Antigen Details

Structure: 14-17 kD protein (134 amino acids), member of the Ly-6 family of GPI linked protein. Ly6 family members share structure homology throughout a distinctive cystein rich protein domain that incorporates O-linked carbohydrates.
Distribution: Ly-6C is expressed primarily on bone marrow myeloid populations, monocytes/macrophages, neutrophils, endothelial cells, and some T cell subsets. Ly-6C is also a marker of memory CD8⁺ T cells.
Cell Type: Endothelial cells, Macrophages, Monocytes, Neutrophils, T cells
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819.
2. Cerwenka A, et al. 1998. J. Immunol. 161:97.
Gene ID: 17067 View all products for this Gene ID
UniProt: View information about Ly-6C on UniProt.org

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Other Formats

View All Ly-6C Reagents Request Custom Conjugation

Description	Clone	Applications
Pacific Blue™ anti-mouse Ly-6C	HK1.4	FC
APC anti-mouse Ly-6C	HK1.4	FC
Purified anti-mouse Ly-6C	HK1.4	FC,IHC-F
Biotin anti-mouse Ly-6C	HK1.4	FC
FITC anti-mouse Ly-6C	HK1.4	FC
Alexa Fluor® 647 anti-mouse Ly-6C	HK1.4	FC
PE anti-mouse Ly-6C	HK1.4	FC
PerCP/Cyanine5.5 anti-mouse Ly-6C	HK1.4	FC
PE/Cyanine7 anti-mouse Ly-6C	HK1.4	FC
Alexa Fluor® 488 anti-mouse Ly-6C	HK1.4	FC
Alexa Fluor® 700 anti-mouse Ly-6C	HK1.4	FC
APC/Cyanine7 anti-mouse Ly-6C	HK1.4	FC
PerCP anti-mouse Ly-6C	HK1.4	FC
Brilliant Violet 570™ anti-mouse Ly-6C	HK1.4	FC
Brilliant Violet 421™ anti-mouse Ly-6C	HK1.4	FC
Brilliant Violet 510™ anti-mouse Ly-6C	HK1.4	FC
Brilliant Violet 605™ anti-mouse Ly-6C	HK1.4	FC
Brilliant Violet 711™ anti-mouse Ly-6C	HK1.4	FC
Purified anti-mouse Ly-6C (Maxpar® Ready)	HK1.4	FC,CyTOF®
Brilliant Violet 785™ anti-mouse Ly-6C	HK1.4	FC
PE/Dazzle™ 594 anti-mouse Ly-6C	HK1.4	FC
APC/Fire™ 750 anti-mouse Ly-6C	HK1.4	FC
TotalSeq™-A0013 anti-mouse Ly-6C	HK1.4	PG
Brilliant Violet 650™ anti-mouse Ly-6C	HK1.4	FC
TotalSeq™-C0013 anti-mouse Ly-6C	HK1.4	PG
TotalSeq™-B0013 anti-mouse Ly-6C	HK1.4	PG
APC/Fire™ 810 anti-mouse Ly-6C Antibody	HK1.4	FC
Spark UV™ 387 anti-mouse Ly-6C	HK1.4	FC
PE/Fire™ 810 anti-mouse Ly-6C	HK1.4	FC
Spark Blue™ 550 anti-mouse Ly-6C (Flexi-Fluor™)	HK1.4	FC
Spark Blue™ 574 anti-mouse Ly-6C (Flexi-Fluor™)	HK1.4	FC
PE/Cyanine5 anti-mouse Ly-6C	HK1.4	FC

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 12/14/2021

For Research Use Only. Not for diagnostic or therapeutic use.

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