Biotin anti-Apo E, 154-162 Antibody, Apo E, 154-162, A17065A

Clone: A17065A (See other available formats)
Regulatory Status: RUO
Other Names: Apolipoprotein E, APO-E, Alzheimer Disease 2, LDLCQ5, APOE, LPG, AD2
Isotype: Mouse IgG2b, κ
Ave. Rating: Submit a Review
Product Citations: publications

A17065A_Biotin_ApoE154-162_Antibody_1_100818 — Western blot of Biotin anti-Apo E, 154-162 antibody (clone A17065A). Lane 1: Molecular weight marker; Lane 2: 40 ng of Apo E2 recombinant human protein; Lane 3: 40 ng of Apo E3 recombinant human protein; Lane 4: 40 ng of Apo E4 recombinant human protein; Lane 5: 40 µg of human plasma. The blot was incubated with 1.0 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP Streptavidin (Cat. No. 405210) for 60 minutes at room temperature. Enhanced chemiluminescence (Cat. No. 426302) was used as the detection system.

Cat #	Size	Price	Save
852805	25 µg	¥29,920
852806	100 µg	¥66,000

Description

Apolipoprotein E (Apo E) belongs to a class of apolipoproteins that mediate the binding, internalization, and catabolism of lipoprotein particles. Apo E is produced by the liver and macrophages in peripheral tissues where it regulates cholesterol metabolism. In the central nervous system, Apo E is primarily produced by astrocytes and plays an important role in transporting cholesterol to neurons via Apo E receptors. Apo E has three alleles (Apo E₂, Apo E₃ and Apo E₄) that differ from each other by only one or two amino acids at positions 112 and 158. Apo E₄ is one of the largest known genetic risk factors for late-onset Alzheimer's disease. Furthermore, Apo E is associated with cardiovascular disease, Lipoprotein Glomerulopathy and Sea-Blue Histiocyte Disease.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: KLH conjugated Apo E peptide encompassing amino acids 154-162.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This antibody cross-reacts with Apo E₂, Apo E₃, and Apo E₄.
RRID: AB_2783425 (BioLegend Cat. No. 852805)
AB_2783425 (BioLegend Cat. No. 852806)

Antigen Details

Structure: Human Apo E is a 299 amino acid protein with a molecular mass of 32 kD, and exists as 3 major alleles, Apo E₂, Apo E₃, and Apo E₄.
Distribution: Tissue sources: Ubiquitously expressed. Highest levels in liver, brain, spleen, adrenal gland, and kidney.
Cellular distribution: Plasma membrane, nucleus, endoplasmic reticulum, golgi apparatus, endosome, and extracellular.
Function: Apo E is involved in lipoprotein metabolism as well as the transport of fat-soluble vitamins, and cholesterol. Apo E also forms complexes with amyloid beta peptides leading to its uptake and degradation by microglia.
Interaction: Tau, amyloid beta
Ligand/Receptor: LDL receptor, SORL1.
Biology Area: Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family: Apolipoproteins
Antigen References: Mahley RW. 2016. Arterioscler Thromb Vasc Biol. 36: 1305-1315.

Riedel BC, et al. 2016 J Steroid Biochem Mol Biol. 160:134-47.

Tai LM, et al. 2016 Acta Neuropathol. 131(5):709-23.

Kim J, et al. 2014. Mol. Cells. 37:767

Holtzman DM, et al. 2012. Cold Spring Harb. Perspect. Med. 2:a006312
Gene ID: 348 View all products for this Gene ID
UniProt: View information about Apo E 154-162 on UniProt.org

Documentation

Reviews

Review This Product

Related Protocols

Western Blotting Protocol

Related Products

Description	Clone	Applications

Related Pages & Pathways

Related FAQs

How many biotin molecules are per antibody structure?: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.

Other Formats

View All Apo E, 154-162 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-Apo E, 154-162	A17065A	WB,Direct ELISA,IHC-P
Biotin anti-Apo E, 154-162	A17065A	WB

Certificate of Analysis

Go To Top Version: 1 Revision Date: 10/09/2018

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.